This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bakke, H.
Right arrow Articles by Haneberg, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bakke, H.
Right arrow Articles by Haneberg, B.

 Previous Article  |  Next Article 

Infection and Immunity, August 2001, p. 5010-5015, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5010-5015.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Meningococcal Outer Membrane Vesicle Vaccine Given Intranasally Can Induce Immunological Memory and Booster Responses without Evidence of Tolerance

Hilde Bakke,1 Kristian Lie,2 Inger Lise Haugen,1 Gro Ellen Korsvold,1 E. Arne Høiby,3 Lisbeth Meyer Næss,1 Johan Holst,1 Ingeborg S. Aaberge,1 Fredrik Oftung,1 and Bjørn Haneberg1,2,*

Department of Vaccinology1 and Department of Bacteriology,3 National Institute of Public Health, N-0403 Oslo, and Department of Microbiology, Institute of Pharmacy, University of Oslo, N-0316 Oslo,2 Norway

Received 28 December 2000/Returned for modification 26 March 2001/Accepted 21 May 2001

We have studied the ability of outer membrane vesicle (OMV) vaccines from Neisseria meningitidis serogroup B to induce vaccine-specific antibody and spleen cell proliferative responses in mice after being administered intranasally (i.n.) and/or subcutaneously (s.c.). A series of four weekly i.n. doses (25 µg) without adjuvant or a single s.c. dose (2.5 µg) with aluminum hydroxide was followed 2 months later by secondary i.n. or s.c. immunizations. After i.n. priming, both immunoglobulin G (IgG) antibody responses in serum, measured by enzyme-linked immunosorbent assay, and IgA antibodies in saliva and extracts of feces were significantly boosted by later i.n. immunizations. The IgG antibody responses in serum were also significantly augmented by secondary s.c. immunization after i.n. as well as s.c. priming. Sera from mice immunized i.n. reached the same level of bactericidal activity as after s.c. immunizations. The s.c. immunizations alone, however, had no effect on mucosal IgA antibody responses, but could prime for booster antibody responses in secretions to later i.n. immunizations. The i.n. immunizations also led to marked OMV-specific spleen cell proliferation in vitro. Both serum antibody responses and spleen cell proliferation were higher after i.n. priming and later s.c. immunizations than after s.c. immunizations alone. There was thus no evidence that i.n. priming had induced immunological tolerance within the B- or T-cell system. Our results indicate that a nonproliferating meningococcal OMV vaccine given i.n. can induce immunological memory and that it may be favorably combined with similar vaccines for injections.

* Corresponding author. Department of Vaccinology, National Institute of Public Health, P.O. Box 4404 Torshov, N-0403 Oslo, Norway. Phone: 47 22 04 25 01. Fax: 47 22 04 23 01. E-mail: bjorn.haneberg{at}folkehelsa.no.

Infection and Immunity, August 2001, p. 5010-5015, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5010-5015.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhu, W., Thomas, C. E., Chen, C.-j., Van Dam, C. N., Johnston, R. E., Davis, N. L., Sparling, P. F. (2005). Comparison of Immune Responses to Gonococcal PorB Delivered as Outer Membrane Vesicles, Recombinant Protein, or Venezuelan Equine Encephalitis Virus Replicon Particles. Infect. Immun. 73: 7558-7568 [Abstract] [Full Text]  
  • Pinto, E. F., Pinheiro, R. O., Rayol, A., Larraga, V., Rossi-Bergmann, B. (2004). Intranasal Vaccination against Cutaneous Leishmaniasis with a Particulated Leishmanial Antigen or DNA Encoding LACK. Infect. Immun. 72: 4521-4527 [Abstract] [Full Text]  
  • Guthrie, T., Wong, S. Y. C., Liang, B., Hyland, L., Hou, S., Hoiby, E. A., Andersen, S. R. (2004). Local and Systemic Antibody Responses in Mice Immunized Intranasally with Native and Detergent-Extracted Outer Membrane Vesicles from Neisseria meningitidis. Infect. Immun. 72: 2528-2537 [Abstract] [Full Text]  
  • Davenport, V., Guthrie, T., Findlow, J., Borrow, R., Williams, N. A., Heyderman, R. S. (2003). Evidence for Naturally Acquired T Cell-Mediated Mucosal Immunity to Neisseria meningitidis. J. Immunol. 171: 4263-4270 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»