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Infection and Immunity, September 2001, p. 5456-5463, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5456-5463.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genetic Immunization with the Region Encoding the alpha -Helical Domain of PspA Elicits Protective Immunity against Streptococcus pneumoniae

Joseph R. Bosarge,1 James M. Watt,2 D. Olga McDaniel,3,4 Edwin Swiatlo,2 and Larry S. McDaniel1,2,3,*

Departments of Microbiology,1 Surgery,3 Medicine,2 and Neurology,4 The University of Mississippi Medical Center, Jackson, Mississippi 39216

Received 12 March 2001/Returned for modification 7 May 2001/Accepted 5 June 2001

Pneumococcal surface protein A (PspA) is a pneumococcal virulence factor capable of eliciting protection against pneumococcal infection in mice. Previous studies have demonstrated that the protection is antibody mediated. Here we examined the ability of pspA to elicit a protective immune response following genetic immunization of mice. Mice were immunized by intramuscular injections with a eukaryotic expression vector encoding the alpha -helical domain of PspA/Rx1. Immunization induced a PspA-specific serum antibody response, and immunized mice survived pneumococcal challenge. Survival and antibody responses occurred in a dose-dependent manner, the highest survival rates being seen with doses of 10 µg or greater. The ability of genetic immunization to elicit cross-protection was demonstrated by the survival of immunized mice challenged with pneumococcal strains differing in capsule and PspA types. Also, immunized mice were protected from intravenous and intratracheal challenges with pneumococci. Similar to the results seen with immunization with PspA, the survival of mice genetically immunized with pspA was antibody mediated. There was no decline in the level of protection 7 months after immunization. These results support the use of genetic immunization to elicit protective immune responses against extracellular pathogens.

* Corresponding author. Mailing address: The University of Mississippi Medical Center, Department of Microbiology, 2500 North State St., Jackson, MS 39216. Phone: (601) 984-6880. Fax: (601) 984-1708. E-mail: LMcDaniel{at}microbio.umsmed.edu.

Infection and Immunity, September 2001, p. 5456-5463, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5456-5463.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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