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Infection and Immunity, September 2001, p. 5892-5898, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5892-5898.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Intermediate Subunit of the Gal/GalNAc Lectin of Entamoeba histolytica Is a Member of a Gene Family Containing Multiple CXXC Sequence Motifs

Xun-Jia Cheng,1 Molly A. Hughes,2 Christopher D. Huston,2 Brendan Loftus,3 Carol A. Gilchrist,2 Lauren A. Lockhart,2 Salil Ghosh,2 Vanessa Miller-Sims,2 Barbara J. Mann,2 William A. Petri Jr.,2,* and Hiroshi Tachibana1

Department of Infectious Diseases, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan1; Departments of Medicine, Microbiology, and Pathology, University of Virginia, Charlottesville, Virginia 22908-13402; and The Institute for Genomic Research, Rockville, Maryland 208503

Received 27 February 2001/Returned for modification 23 April 2001/Accepted 4 June 2001

Killing by Entamoeba histolytica requires parasite adherence to host galactose- and N-acetyl-D-galactosamine (Gal/GalNAc)-containing cell surface receptors. A 260-kDa heterodimeric E. histolytica Gal/GalNAc lectin composed of heavy (Hgl) and light (Lgl) subunits has been previously described. Here we present the cloning and characterization of Igl, a 150-kDa intermediate subunit of the Gal/GalNAc lectin. Igl, Hgl, and Lgl colocalized on the surface membrane of trophozoites. Two unlinked copies of genes encoding Igl shared 81% amino acid sequence identity (GenBank accession no. AF337950 and AF337951). They encoded cysteine-rich proteins with amino- and carboxy-terminal hydrophobic signal sequences characteristic of glycosylphosphatidylinositol (GPI)-anchored membrane proteins. The igl genes lacked carbohydrate recognition domains but were members of a large family of amebic genes containing CXXC and CXC motifs. These data indicate that Igl is part of the parasite's multimolecular Gal/GalNAc adhesin required for host interaction.

* Corresponding author. Present address: Room 2115 MR4, Division of Infectious Diseases, P.O. Box 801340, University of Virginia Health System, Charlottesville, VA 22908-1340. Phone: (434) 924-5621. Fax: (434) 924-0075. E-mail: wap3g{at}virginia.edu.

Infection and Immunity, September 2001, p. 5892-5898, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5892-5898.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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