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Infection and Immunity, September 2001, p. 5905-5907, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5905-5907.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Proteomics Reveals Open Reading Frames in Mycobacterium tuberculosis H37Rv Not Predicted by Genomics

Peter R. Jungblut,1,* Eva-Christina Müller,2 Jens Mattow,3 and Stefan H. E. Kaufmann3

Core Facility for Protein Analysis1 and Department of Immunology,3 Max Planck Institute for Infection Biology, and Protein Chemistry, Max Delbrück Center,2 Berlin, Germany

Received 23 February 2001/Returned for modification 27 March 2001/Accepted 25 May 2001

Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser desorption ionization and nano-electrospray mass spectrometry.

* Corresponding author. Mailing address: Max Planck Institute for Infection Biology, Core Facility for Protein Analysis, Schumannstr. 21-22, D-10117 Berlin, Germany. Phone: 49-30-28460133. Fax: 49-30-2846-0507. E-mail: jungblut{at}mpiib-berlin.mpg.de.

Infection and Immunity, September 2001, p. 5905-5907, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5905-5907.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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