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Infection and Immunity, September 2001, p. 5921-5924, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5921-5924.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Demonstration of Polysaccharide Capsule in Campylobacter jejuni Using Electron Microscopy

Andrey V. Karlyshev, Maria V. McCrossan, and Brendan W. Wren*

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, London WC1E 7HT, United Kingdom

Received 8 February 2001/Returned for modification 10 April 2001/Accepted 30 May 2001

Recently, we reported that Campylobacter jejuni, an important gastrointestinal pathogen, has the genetic determinants to produce a capsular polysaccharide (Karlyshev et al., Mol. Microbiol. 35:529-541, 2000). Despite these data, the presence of a capsule in these bacteria has remained controversial. In this study we stain C. jejuni cells with the cationic dye Alcian blue and demonstrate for the first time by electron microscopy that C. jejuni cells produce a polysaccharide capsule that is retained in the coccoid form but is absent in a kpsM mutant.

* Corresponding author. Mailing address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel Street, London WC1E 7HT, United Kingdom. Phone:: 44 (0) 207 927 2288. E-mail: brendan.wren{at}lshtm.ac.uk.

Infection and Immunity, September 2001, p. 5921-5924, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5921-5924.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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