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Infection and Immunity, September 2001, p. 5936-5939, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5936-5939.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of the Secreted MPT53 Antigen of Mycobacterium tuberculosis†

Sadie Johnson,1 PierNatale Brusasca,1 Konstantin Lyashchenko,1 John S. Spencer,2 Harald G. Wiker,3 Pablo Bifani,1 Elena Shashkina,1 Barry Kreiswirth,1 Morten Harboe,4 Neil Schluger,5 Manuel Gomez,1 and Maria Laura Gennaro1,*

Public Health Research Institute, New York, New York, 100161; Colorado State University, Fort Collins, Colorado 805232; National Institute of Public Health, N-0403 Oslo,3 and Institute of Immunology, The National Hospital, N-0027 Oslo,4 Norway; and Columbia University College of Physicians and Surgeons, New York, New York, 100325

Received 15 December 2000/Returned for modification 6 March 2001/Accepted 22 May 2001

MPT53 is a secreted protein of Mycobacterium tuberculosis. Southern transfer and hybridization showed mpt53 to be conserved in the M. tuberculosis complex and to have homology with DNA from Mycobacterium avium and other nontuberculous mycobacteria. However, anti-MPT53 polyclonal antibodies detected no antigen in the culture filtrates of M. avium and other nontuberculous mycobacteria. MPT53 of M. tuberculosis induced strong, tuberculosis-specific antibody responses in guinea pigs but induced no delayed-type hypersensitivity. Involvement in immune responses during human tuberculosis was very modest.

* Corresponding author. Mailing address: Public Health Research Institute, 455 First Ave, New York, NY 10016. Phone: (212) 578-0844. Fax: (212) 578-0804. E-mail: gennaro{at}phri.nyu.edu.

dagger Report no. 80 of the Public Health Research Institute TB Center.

Infection and Immunity, September 2001, p. 5936-5939, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5936-5939.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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