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Infection and Immunity, January 2002, p. 407-411, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.407-411.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Influence of the Length of the Lipooligosaccharide {alpha} Chain on Its Sialylation in Neisseria meningitidis

Chao-Ming Tsai,* George Kao, and Peixuan Zhu

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics, Food and Drug Administration, Bethesda, Maryland 20892

Received 23 August 2001/ Accepted 11 October 2001

The sialylation of lipooligosaccharide (LOS) in Neisseria meningitidis plays a role in the resistance of the organism to killing by normal human serum. The length of the {alpha} chain extending out from the heptose I [Hep (I)] moiety of LOS influenced sialylation of N. meningitidis LOS in vitro and in vivo. The {alpha} chain required a terminal Gal and a trisaccharide or longer oligosaccharide to serve as an acceptor for sialylation. The disaccharide lactose (Galß1-4Glc) in the {alpha} chain of immunotype L8 LOS could not function as an acceptor for the sialyltransferase, probably due to steric hindrance imposed by the neighboring Hep (II) with phosphorylethanolamine and another group attached.

* Corresponding author. Mailing address: Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics, Food and Drug Administration, HFM-428, 1401 Rockville Pike, Rockville, MD 20852. Phone: (301) 496-1947. Fax: (301) 402-2776. E-mail: tsai{at}cber.fda.gov.

Editor: E. I. Tuomanen

Infection and Immunity, January 2002, p. 407-411, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.407-411.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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