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Infection and Immunity, November 2002, p. 6242-6250, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6242-6250.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

DNA Sequence and Mutational Analyses of the pVir Plasmid of Campylobacter jejuni 81-176

David J. Bacon,1 Richard A. Alm,2 Lan Hu,1 Thomas E. Hickey,1 Cheryl P. Ewing,1 Roger A. Batchelor,1 Trevor J. Trust,2 and Patricia Guerry1*

Naval Medical Research Center, Silver Spring, Maryland 20910,1 AstraZeneca R & D Boston, Waltham, Massachusetts 024512

Received 25 February 2002/ Returned for modification 23 April 2002/ Accepted 29 July 2002

The circular pVir plasmid of Campylobacter jejuni strain 81-176 was determined to be 37,468 nucleotides in length with a G+C content of 26%. A total of 83% of the plasmid represented coding information, and all but 2 of the 54 predicted open reading frames were encoded on the same DNA strand. There were seven genes on the plasmid in a continguous region of 8.9 kb that encoded orthologs of type IV secretion proteins found in Helicobacter pylori, including four that have been described previously (D. J. Bacon, R. A. Alm, D. H. Burr, L. Hu, D. J. Kopecko, C. P. Ewing, T. J. Trust, and P. Guerry, Infect. Immun. 68:4384-4390, 2000). There were seven other pVir-encoded proteins that showed significant similarities to proteins encoded by the plasticity zones of either H. pylori J99 or 26695. Mutational analyses of 19 plasmid genes identified 5 additional genes that affect in vitro invasion of intestinal epithelial cells. These included one additional gene encoding a component of a type IV secretion system, an ortholog of Cj0041 from the chromosome of C. jejuni NCTC 11168, two Campylobacter plasmid-specific genes, and an ortholog of HP0996 from the plasticity zone of H. pylori 26695.

* Corresponding author. Mailing address: Enteric Diseases Program, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910-7500. Phone: (301) 319-7662. Fax: (301) 319-7679. E-mail: guerryp{at}nmrc.navy.mil.

Editor: V. J. DiRita

Infection and Immunity, November 2002, p. 6242-6250, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6242-6250.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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