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Infection and Immunity, November 2002, p. 6355-6364, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6355-6364.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Shigella flexneri DegP Facilitates IcsA Surface Expression and Is Required for Efficient Intercellular Spread

Georgiana E. Purdy, Mei Hong,{dagger} and Shelley M. Payne*

Institute for Cellular and Molecular Biology and Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712

Received 10 May 2002/ Returned for modification 9 August 2002/ Accepted 14 August 2002

A degP mutant of Shigella flexneri was identified in a screen for insertion mutants that invaded cultured cells but did not form wild-type plaques in monolayers. The degP mutant SM1100 invaded Henle cells at wild-type levels and induced apoptosis in macrophages but formed smaller plaques than those formed by wild-type S. flexneri in confluent monolayers of Henle and Caco-2 cells. The proportion of SM1100 bacteria with IcsA localized to the bacterial pole, a process required for actin polymerization into actin "tails," was reduced compared to results with wild-type bacteria. The reduction in proper IcsA localization may account for the reduced plaque size of the degP mutant. Although DegP is a protease, the protease activity of S. flexneri DegP was not required for IcsA localization or the formation of plaques in Henle cell monolayers. DegP was also required for efficient polar IcsA localization in E. coli expressing icsA. In addition, the growth or survival of SM1100 was compromised compared to that of the wild type at elevated temperatures and in acidic conditions.

* Corresponding author. Mailing address: University of Texas, Section of Molecular Genetics and Microbiology, Austin, TX 78712-1095. Phone: (512) 471-9258. Fax: (512) 471-7088. E-mail: payne{at}mail.utexas.edu.

Editor: J. T. Barbieri

{dagger} Present address: Chiron Corporation, Emeryville, CA 94608.

Infection and Immunity, November 2002, p. 6355-6364, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6355-6364.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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