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Infection and Immunity, November 2002, p. 6464-6467, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6464-6467.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The N-Terminal Domain of RTX Toxin ApxI of Actinobacillus pleuropneumoniae Elicits Protective Immunity in Mice

J. N. Seah,1 J. Frey,2 and J. Kwang1*

Laboratory of Animal Health Biotechnology, Temasek Life Sciences Laboratory, The National University of Singapore, Singapore 117604, Singapore,1 Institute for Veterinary Bacteriology, University of Berne, CH-3012 Berne, Switzerland2

Received 3 June 2002/ Returned for modification 9 July 2002/ Accepted 29 July 2002

We expressed three Actinobacillus pleuropneumoniae ApxI deletion derivatives to map the domain that could induce protective immunity. Antiserum to ApxI N-terminal covered by residues 40 to 380 was found to neutralize ApxI hemolytic activity but not ApxIII cytotoxicity. When used as a subunit vaccine in mice, this recombinant N-terminal fragment elicited protection against lethal infection with heterologous A. pleuropneumoniae serovars.

* Corresponding author. Mailing address: Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604, Singapore. Phone: 65-8727473. Fax: 65-8727007. E-mail: Kwang{at}tll.org.sg.

Editor: J. T. Barbieri

Infection and Immunity, November 2002, p. 6464-6467, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6464-6467.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Whitby, P. W., VanWagoner, T. M., Taylor, A. A., Seale, T. W., Morton, D. J., LiPuma, J. J., Stull, T. L. (2006). Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization. J Med Microbiol 55: 11-21 [Abstract] [Full Text]  


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