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Infection and Immunity, December 2002, p. 7120-7125, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7120-7125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Construction of an Actinobacillus pleuropneumoniae Serotype 2 Prototype Live Negative-Marker Vaccine

Walaiporn Tonpitak,1,{dagger} Nina Baltes,1 Isabel Hennig-Pauka,2 and Gerald F. Gerlach1*

Institut für Mikrobiologie und Tierseuchen,1 Klinik für Kleine Klauentiere, Tieraerztliche Hochschule Hannover, 30173 Hanover, Germany2

Received 11 June 2002/ Returned for modification 22 July 2002/ Accepted 4 September 2002

Deletions were introduced into the ureC and apxIIA genes of an Actinobacillus pleuropneumoniae serotype 2 strain by homologous recombination and counterselection. The double-mutant contains no foreign DNA, is highly attenuated, protects pigs from homologous challenge upon a single aerosol application, and facilitates the serological discrimination of immunized and infected herds.

* Corresponding author. Mailing address: Tieraerztliche Hochschule, Hannover Institut fuer Mikrobiologie und Tierseuchen, D-30173 Hanover, Germany. Phone: 49-511-856-7598. Fax: 49-511-856-7697. E-mail: gfgerlach{at}gmx.de.

Editor: B. B. Finlay

{dagger} Present address: Department of Microbiology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.

Infection and Immunity, December 2002, p. 7120-7125, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7120-7125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Jacobsen, I., Hennig-Pauka, I., Baltes, N., Trost, M., Gerlach, G.-F. (2005). Enzymes Involved in Anaerobic Respiration Appear To Play a Role in Actinobacillus pleuropneumoniae Virulence. Infect. Immun. 73: 226-234 [Abstract] [Full Text]  
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