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Infection and Immunity, February 2002, p. 649-654, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.649-654.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mycoplasma pneumoniae Induces Chronic Respiratory Infection, Airway Hyperreactivity, and Pulmonary Inflammation: a Murine Model of Infection-Associated Chronic Reactive Airway Disease

Robert D. Hardy,1* Hasan S. Jafri,1 Kurt Olsen,1 Jeanine Hatfield,1 Janie Iglehart,1 Beverly B. Rogers,1 Padma Patel,2 Gail Cassell,2 George H. McCracken,1 and Octavio Ramilo1

Departments of Pediatric Infectious Diseases and Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390,1 Diagnostic Mycoplasma Laboratory, University of Alabama, Birmingham, Alabama 352032

Received 23 July 2001/ Returned for modification 21 September 2001/ Accepted 19 November 2001

Because chronic Mycoplasma pneumoniae respiratory infection is hypothesized to play a role in asthma, the potential of M. pneumoniae to establish chronic respiratory infection with associated pulmonary disease was investigated in a murine model. BALB/c mice were intranasally inoculated once with M. pneumoniae and examined at 109, 150, 245, 368, and 530 days postinoculation. M. pneumoniae was detected in bronchoalveolar lavage fluid by culture or PCR in 70 and 22% of mice at 109 and 530 days postinoculation, respectively. Lung histopathology was normal up to 368 days postinoculation. At 530 days, however, 78% of the mice inoculated with M. pneumoniae demonstrated abnormal histopathology characterized by peribronchial and perivascular mononuclear infiltrates. A mean histopathologic score (HPS) at 530 days of 5.1 was significantly greater (P < 0.01) than that for controls (HPS score of 0). Serum anti-M. pneumoniae immunoglobulin G was detectable in all of the mice inoculated with M. pneumoniae and was inversely correlated with HPS (r = -0.95, P = 0.01) at 530 days postinoculation. Unrestrained whole-body plethysmography measurement of enhanced pause revealed significantly elevated airway methacholine reactivity in M. pneumoniae-inoculated mice compared with that in controls at 245 days (P = 0.03) and increased airway obstruction at 530 days (P = 0.01). Murine M. pneumoniae respiratory infection can lead to chronic pulmonary disease characterized by airway hyperreactivity, airway obstruction, and histologic inflammation.

* Corresponding author. Mailing address: Department of Pediatrics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9063. Phone: (214) 648-3720. Fax: (214) 648-2961. E-mail: rhardy{at}mednet.swmed.edu.

Editor: E. I. Tuomanen

Infection and Immunity, February 2002, p. 649-654, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.649-654.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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