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Infection and Immunity, February 2002, p. 878-888, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.878-888.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of Biofilms Formed by Candida albicans and Candida parapsilosis on Bioprosthetic Surfaces

D. M. Kuhn,1,2 J. Chandra,1,3 P. K. Mukherjee,1,3 and M. A. Ghannoum1,3*

Case Western Reserve University,1 Division of Infectious DiseasesDepartment of Medicine,,2 Department of Dermatology, University Hospitals of Cleveland, Cleveland, Ohio 441063

Received 8 June 2001/ Returned for modification 9 October 2001/ Accepted 30 October 2001

Little is known about fungal biofilms, which may cause infection and antibiotic resistance. In this study, biofilm formation by different Candida species, particularly Candida albicans and C. parapsilosis, was evaluated by using a clinically relevant model of Candida biofilm on medical devices. Candida biofilms were allowed to form on silicone elastomer and were quantified by tetrazolium (XTT) and dry weight (DW) assays. Formed biofilm was visualized by using fluorescence microscopy and confocal scanning laser microscopy with Calcofluor White (Sigma Chemical Co., St. Louis, Mo.), concanavalin A-Alexafluor 488 (Molecular Probes, Eugene, Oreg.), and FUN-1 (Molecular Probes) dyes. Although minimal variations in biofilm production among invasive C. albicans isolates were seen, significant differences between invasive and noninvasive isolates (P < 0.001) were noted. C. albicans isolates produced more biofilm than C. parapsilosis, C. glabrata, and C. tropicalis isolates, as determined by DW assays (P was <0.001 for all comparisons) and microscopy. Interestingly, noninvasive isolates demonstrated a higher level of XTT activity than invasive isolates. On microscopy, C. albicans biofilms had a morphology different from that of other species, consisting of a basal blastospore layer with a dense overlying matrix composed of exopolysaccharides and hyphae. In contrast, C. parapsilosis biofilms had less volume than C. albicans biofilms and were comprised exclusively of clumped blastospores. Unlike planktonically grown cells, Candida biofilms rapidly (within 6 h) developed fluconazole resistance (MIC, >128 µg/ml). Importantly, XTT and FUN-1 activity showed biofilm cells to be metabolically active. In conclusion, our data show that C. albicans produces quantitatively larger and qualitatively more complex biofilms than other species, in particular, C. parapsilosis.

* Corresponding author. Mailing address: Center for Medical Mycology, University Hospitals of Cleveland, 11100 Euclid Ave., Cleveland, OH 44106. Phone: (216) 844-8580. Fax: (216) 844-1076. E-mail: mag3{at}po.cwru.edu.

Editor: T. R. Kozel

Infection and Immunity, February 2002, p. 878-888, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.878-888.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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