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Infection and Immunity, February 2002, p. 974-980, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.974-980.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Adherence of Burkholderia pseudomallei Cells to Cultured Human Epithelial Cell Lines Is Regulated by Growth Temperature

Nat F. Brown, Justin A. Boddey, Cameron P. Flegg, and Ifor R. Beacham*

School of Health Science, Griffith University—Gold Coast Campus, Gold Coast, Queensland, Australia

Received 14 May 2001/ Returned for modification 16 July 2001/ Accepted 27 September 2001

We have investigated the adherence of Burkholderia pseudomallei, cultured under a number of different conditions, to six human epithelial cell lines. While several complex medium compositions had relatively little effect on adherence, growth at 30°C was found to significantly increase adherence to all cell lines relative to that of cultures grown at 37°C (P < 0.001).


* Corresponding author. Mailing address: School of Health Science, Griffith University—Gold Coast Campus, PMB 50 Gold Coast Mail Centre, QLD 4217, Australia. Phone: 61 7 5552 8185. Fax: 61 7 5552 8908. E-mail: i.beacham{at}mailbox.gu.edu.au.

Editor: V. J. DiRita


Infection and Immunity, February 2002, p. 974-980, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.974-980.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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