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Infection and Immunity, March 2002, p. 1319-1325, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1319-1325.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Contribution of Fibronectin-Binding Protein to Pathogenesis of Streptococcus suis Serotype 2

Astrid de Greeff,1,2* Herma Buys,2 Robin Verhaar,2 Janny Dijkstra,2 Loek van Alphen,1,3 and Hilde E. Smith2

Department of Medical Microbiology, University of Amsterdam, 1100 DD Amsterdam,1 Division of Infectious Diseases and Food Chain Quality, Cluster of Endemic Diseases, Institute for Animal Science and Health, 8200 AB Lelystad,2 Laboratory for Vaccine Research, RIVM, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands3

Received 19 July 2001/ Returned for modification 27 August 2001/ Accepted 15 November 2001

In the present study we investigated the role of the fibronectin (FN)- and fibrinogen (FGN)-binding protein (FBPS) in the pathogenesis of Streptococcus suis serotype 2 in piglets. The complete gene encoding FBPS from S. suis serotype 2 was cloned in Escherichia coli and sequenced. The occurrence of the gene in various serotypes was analyzed by hybridization studies. The FBPS protein was expressed in E. coli and purified, and binding to human FN and FGN was demonstrated. The induction of antibodies in piglets was studied upon infection. An isogenic mutant unable to produce FBPS was constructed, and the levels of virulence of the wild-type and mutant strains were compared in a competitive infection model in young piglets. Organ cultures showed that FBPS was not required for colonization of the tonsils but that FBPS played a role in the colonization of the specific organs involved in an S. suis infection. Therefore, the FBPS mutant was considered as an attenuated mutant.

* Corresponding author. Mailing address: Division of Infectious Diseases and Food Chain Quality, Cluster of Endemic Diseases, Institute for Animal Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Phone: 31 320 238403. Fax: 31 320 238153. E-mail: a.degreeff{at}id.wag-ur.nl.

Infection and Immunity, March 2002, p. 1319-1325, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1319-1325.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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