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Infection and Immunity, March 2002, p. 1403-1409, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1403-1409.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Type III Secretion of Salmonella enterica Serovar Typhimurium Translocated Effectors and SseFG{dagger}

Imke Hansen-Wester,1 Bärbel Stecher,2 and Michael Hensel1*

Lehrstuhl für Bakteriologie, Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, LMU München, Munich, and Institut für Klinische Mikrobiologie, Immunologie und Hygiene, FAU Erlangen—Nürnberg, Erlangen, Germany1

Received 23 August 2001/ Accepted 13 December 2001

The type III secretion system (TTSS) encoded by Salmonella enterica serovar Typhimurium pathogenicity island 2 (SPI2) is employed by Salmonella enterica for interaction with host cells during the intracellular phase of pathogenesis. This TTSS secretes a set of SPI2-encoded proteins in vitro and translocates Salmonella serovar Typhimurium translocated effectors (STE) that are encoded by genes outside of SPI2 into host cells. Using an epitope-tagging approach, we analyzed secretion of proteins by the TTSS of SPI2 and identified SseF and SseG as further secreted substrate proteins. Three members of the STE family, SifA, SifB, and SseJ, were secreted under conditions that also induce secretion of SPI2-encoded substrate proteins.

* Corresponding author. Mailing address: Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Friedrich-Alexander-Universität Erlangen—Nürnberg, Wasserturmstr. 3-5, D-91054 Erlangen, Germany. Phone: 49 9131 85 23640. Fax: 49 9131 85 22531. E-mail: hensel{at}mikrobio.med.uni-erlangen.de

{dagger} Michael Hensel dedicates this article to Karlheinz Altendorf (Osnabrück) on the occasion of his 60th birthday.

Infection and Immunity, March 2002, p. 1403-1409, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1403-1409.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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