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Infection and Immunity, March 2002, p. 1481-1487, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1481-1487.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of Surfactant Protein D in the Human Gastric Mucosa and during Helicobacter pylori Infection

Emma Murray,1 Wafa Khamri,2 Marjorie M. Walker,2 Paul Eggleton,1 Anthony P. Moran,3 John A. Ferris,3 Susanne Knapp,3 Q. Najma Karim,3 Mulegata Worku,3 Peter Strong,1 Kenneth B. M. Reid,1 and Mark R. Thursz3*

MRC Immunochemistry Unit, Oxford,1 Faculty of Medicine, Imperial College of Science, Technology and Medicine, St. Mary's Hospital Campus, London, United Kingdom,2 Department of Microbiology, National University of Ireland, Galway, Ireland3

Received 18 January 2001/ Returned for modification 11 April 2001/ Accepted 15 October 2001

Helicobacter pylori establishes persistent infection of gastric mucosa with diverse clinical outcomes. The innate immune molecule surfactant protein D (SP-D) binds selectively to microorganisms, inducing aggregation and phagocytosis. In this study, we demonstrated the expression of SP-D in gastric mucosa by reverse transcription-PCR and immuohistochemical analysis. SP-D is present at the luminal surface and within the gastric pits, with maximal expression at the surface. Levels of expression are significantly increased in H. pylori-associated gastritis compared to those in the normal mucosa. Immunofluorescence microscopy was used to demonstrate binding and agglutination of H. pylori by SP-D in a lectin-specific manner. These activities resulted in a 50% reduction in the motility of H. pylori, as judged on the basis of curvilinear velocity measured by using a Hobson BacTracker. Lipopolysaccharides extracted from three H. pylori strains were shown to bind SP-D in a concentration-dependent manner, and there was marked variation in the avidity of binding among the strains. SP-D may therefore play a significant role in the innate immune response to H. pylori infection.

* Corresponding author. Mailing address: Faculty of Medicine, Imperial College of Science, Technology and Medicine, St. Mary's Hospital Campus, Norfolk Place, London W2 1NY, United Kingdom. Phone: 44 207 594 3851. Fax: 44 207 706 9161. E-mail: m.thursz{at}ic.ac.uk.

Infection and Immunity, March 2002, p. 1481-1487, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1481-1487.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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