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Infection and Immunity, March 2002, p. 1604-1608, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1604-1608.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Influence of the Alternative {sigma}28 Factor on Virulence and Flagellum Expression of Legionella pneumophila

Klaus Heuner,* Claudia Dietrich, Carina Skriwan, Michael Steinert, and Jörg Hacker

Institut für Molekulare Infektionsbiologie, Universität Würzburg, 97070 Würzburg, Germany

Received 14 June 2001/ Returned for modification 15 August 2001/ Accepted 20 November 2001

The fliA gene of Legionella pneumophila encoding the alternative {sigma}28 factor was inactivated by introducing a kanamycin resistance cassette. Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin. Reporter gene assays indicated that the flaA promoter is not active in the fliA mutant strain. The fliA mutant strain multiplied less effectively in coculture with amoebae than the wild-type strain and was not able to replicate in coculture with Dictyostelium discoideum.

* Corresponding author. Mailing address: Institut für Molekulare Infektionsbiologie, Universität Würzburg, Röntgenring 11, 97070 Würzburg, Germany. Phone: 49-931312577. Fax: 49-931312578. E-mail: klaus.heuner{at}mail.uni-wuerzburg.de.

Infection and Immunity, March 2002, p. 1604-1608, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1604-1608.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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