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Infection and Immunity, March 2002, p. 1631-1634, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1631-1634.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis

Stephan Köhler,1* Euloge Ekaza,1 Jean-Yves Paquet,2 Karl Walravens,2 Jacques Teyssier,1 Jacques Godfroid,2 and Jean-Pierre Liautard1

Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France,1 Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium2

Received 14 September 2001/ Returned for modification 29 November 2001/ Accepted 21 December 2001

The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37°C, with a thermal cutoff at 39°C. However, the constitutive dnaK mutant, which showed high sensitivity to H2O2-mediated stress, failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments to our hypothesis that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B. suis.

* Corresponding author. Mailing address: INSERM U-431, Université Montpellier II, C.P. 100, Pl. E. Bataillon, 34095 Montpellier, France. Phone: (33) 4 67 14 42 38. Fax: (33) 4 67 14 33 38. E-mail: kohler{at}crit.univ-montp2.fr.

Infection and Immunity, March 2002, p. 1631-1634, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1631-1634.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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