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Infection and Immunity, March 2002, p. 1653-1656, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1653-1656.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Recombinant Carboxy-Terminal Domain of the Protective Antigen of Bacillus anthracis Protects Mice against Anthrax Infection

Helen C. Flick-Smith, Nicola J. Walker, Paula Gibson, Helen Bullifent, Sarah Hayward, Julie Miller, Richard W. Titball, and E. Diane Williamson*

Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom

Received 25 July 2001/ Returned for modification 26 September 2001/ Accepted 12 November 2001

The immunogenicity and protective efficacy of overlapping regions of the protective antigen (PA) polypeptide, cloned and expressed as glutathione S-transferase fusion proteins, have been assessed. Results show that protection can be attributed to individual domains and imply that it is domain 4 which contains the dominant protective epitopes of PA.

* Corresponding author. Mailing address: Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom. Phone: 01980 613895. Fax: 01980 614307. E-mail: HCFSmith{at}dstl.gov.uk.

Infection and Immunity, March 2002, p. 1653-1656, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1653-1656.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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