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Infection and Immunity, April 2002, p. 1991-1996, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.1991-1996.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Involvement of PhoP-PhoS Homologs in Enterococcus faecalis Virulence

Fang Teng,1,2 Ling Wang,1 Kavindra V. Singh,2,3 Barbara E. Murray,1,2,3 and George M. Weinstock1,2*

Department of Microbiology and Molecular Genetics,1 Division of Infectious Disease, Department of Medicine,3 Center for the Study of Emerging and Reemerging Pathogens, University of Texas Houston Medical School, Houston, Texas 770302

Received 29 August 2001/ Returned for modification 4 December 2001/ Accepted 18 January 2002

Eleven PhoP-PhoS homolog pairs were identified by searching the Enterococcus faecalis V583 genome sequence database at The Institute for Genomic Research with the Bacillus subtilis PhoP-PhoS sequences. Each pair appears to be a potential two-component system composed of a response regulator and a sensor kinase. Seven of the homologs were disrupted in E. faecalis strain OG1RF. TX10293, a mutant disrupted in one of these genes (etaR, the first gene of the gene pair designated etaRS), showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. The predicted EtaR protein sequence showed greatest similarity to LisR of Listeria monocytogenes (77%) and CsrR of Streptococcus pyogenes (70%); EtaS is 53% similar to LisK and 54% similar to CsrS. When grown in vitro, the TX10293 mutant was more sensitive to low pH (pH 3.4) and more resistant to high temperature (55°C) than wild-type OG1RF. In conclusion, many potential two-component systems are identified for E. faecalis, one of which, EtaRS, was shown to be involved in stress response and virulence.

* Corresponding author. Mailing address: Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Alkek Building, N1519, Houston, Texas 77030. Phone: (713) 798-6539. Fax: (713) 798-5741. E-mail: gwstock{at}bcm.tmc.edu.

Editor: E. I. Tuomanen

Infection and Immunity, April 2002, p. 1991-1996, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.1991-1996.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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