This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y. L.
Right arrow Articles by Leung, K. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y. L.
Right arrow Articles by Leung, K. Y.

 Previous Article  |  Next Article 

Infection and Immunity, May 2002, p. 2326-2335, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2326-2335.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Aeromonas hydrophila PPD134/91 Genes Involved in O-Antigen and Capsule Biosynthesis

Y. L. Zhang,1 E. Arakawa,2 and K. Y. Leung1,3*

Department of Biological Sciences, Faculty of Science,1 Tropical Marine Science Institute, The National University of Singapore, Singapore 117543,3 Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan 162-86402

Received 10 July 2001/ Returned for modification 14 September 2001/ Accepted 3 January 2002

The sequences of the O-antigen and capsule gene clusters of the virulent Aeromonas hydrophila strain PPD134/91 were determined. The O-antigen gene cluster is 17,296 bp long and comprises 17 genes. Seven pathway genes for the synthesis of rhamnose and mannose, six transferase genes, one O unit flippase gene, and one O-antigen chain length determinant gene were identified by amino acid sequence similarity. PCR and Southern blot analysis were performed to survey the distribution of these 17 genes among 11 A. hydrophila strains of different serotypes. A. hydrophila PPD134/91 might belong to serotype O:18, as represented by JCM3980; it contained all the same O-antigen genes as JCM3980 (97 to 100% similarity at the DNA and amino acid levels). The capsule gene cluster of A. hydrophila PPD134/91 is 17,562 bp long and includes 13 genes, which were assembled into three distinct regions similar to those of the group II capsule gene cluster of Escherichia coli and other bacteria. Regions I and III contained four and two capsule transport genes, respectively. Region II had five genes which were highly similar to capsule synthesis pathway genes found in other bacteria. Both the purified O-antigen and capsular polysaccharides increased the ability of the avirulent A. hydrophila strain PPD35/85 to survive in naïve tilapia serum. However, the purified surface polysaccharides had no inhibitory effect on the adhesion of A. hydrophila PPD134/91 to carp epithelial cells.

* Corresponding author. Mailing address: Department of Biological Sciences, Faculty of Science, The National University of Singapore, Science Dr. 4, Singapore, 117543. Phone: (65) 68747835. Fax: (65) 67792486. E-mail: dbslky{at}nus.edu.sg.

Editor: R. N. Moore

Infection and Immunity, May 2002, p. 2326-2335, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2326-2335.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jimenez, N., Canals, R., Salo, M. T., Vilches, S., Merino, S., Tomas, J. M. (2008). The Aeromonas hydrophila wb*O34 Gene Cluster: Genetics and Temperature Regulation. J. Bacteriol. 190: 4198-4209 [Abstract] [Full Text]  
  • Silver, A. C., Rabinowitz, N. M., Kuffer, S., Graf, J. (2007). Identification of Aeromonas veronii Genes Required for Colonization of the Medicinal Leech, Hirudo verbana. J. Bacteriol. 189: 6763-6772 [Abstract] [Full Text]  
  • Canals, R., Jimenez, N., Vilches, S., Regue, M., Merino, S., Tomas, J. M. (2006). The UDP N-Acetylgalactosamine 4-Epimerase Gene Is Essential for Mesophilic Aeromonas hydrophila Serotype O34 Virulence. Infect. Immun. 74: 537-548 [Abstract] [Full Text]  
  • Yu, H. B., Zhang, Y. L., Lau, Y. L., Yao, F., Vilches, S., Merino, S., Tomas, J. M., Howard, S. P., Leung, K. Y. (2005). Identification and Characterization of Putative Virulence Genes and Gene Clusters in Aeromonas hydrophila PPD134/91. Appl. Environ. Microbiol. 71: 4469-4477 [Abstract] [Full Text]  
  • Vilches, S., Urgell, C., Merino, S., Chacon, M. R., Soler, L., Castro-Escarpulli, G., Figueras, M. J., Tomas, J. M. (2004). Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain. Appl. Environ. Microbiol. 70: 6914-6919 [Abstract] [Full Text]  
  • Yu, H. B., Rao, P. S. S., Lee, H. C., Vilches, S., Merino, S., Tomas, J. M., Leung, K. Y. (2004). A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis. Infect. Immun. 72: 1248-1256 [Abstract] [Full Text]  
  • Braschler, T. R., Merino, S., Tomas, J. M., Graf, J. (2003). Complement Resistance Is Essential for Colonization of the Digestive Tract of Hirudo medicinalis by Aeromonas Strains. Appl. Environ. Microbiol. 69: 4268-4271 [Abstract] [Full Text]  
  • Zhang, Y. L., Lau, Y. L., Arakawa, E., Leung, K. Y. (2003). Detection and genetic analysis of group II capsules in Aeromonas hydrophila. Microbiology 149: 1051-1060 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»