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Infection and Immunity, May 2002, p. 2694-2699, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2694-2699.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genomic Analysis of the F3031 Brazilian Purpuric Fever Clone of Haemophilus influenzae Biogroup Aegyptius by PCR-Based Subtractive Hybridization{dagger}

Laura M. Smoot,,§ Deanna D. Franke,,{dagger} Glen McGillivary, and Luis A. Actis*

Department of Microbiology, Miami University, Oxford, Ohio

Received 7 September 2001/ Returned for modification 4 December 2001/ Accepted 30 January 2002

PCR-based subtractive genome hybridization produced clones harboring inserts present in Brazilian purpuric fever (BPF) prototype strain F3031 but absent in noninvasive Haemophilus influenzae biogroup aegyptius isolate F1947. Some of these inserts have no matches in the GenBank database, while others are similar to genes encoding either known or hypothetical proteins. One insert represents a 2.3-kb locus with similarity to a Thermotoga maritima hypothetical protein, while another is part of a 7.6-kb locus that contains predicted genes encoding hypothetical, phage-related, and carotovoricin Er-like proteins. The presence of DNA related to these loci is variable among BPF isolates and nontypeable H. influenzae strains, while neither of them was detected in strains of types a to f. The data indicate that BPF-causing strain F3031 harbors unique chromosomal regions, most of which appear to be acquired from unrelated microbial sources.

* Corresponding author. Mailing address: Department of Microbiology, Miami University, 40 Pearson Hall, Oxford, OH 45056. Phone: (513) 529-5424. Fax: (513) 529-2431. E-mail: actisla{at}muohio.edu.

{ddagger} Present address: Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840.

Editor: V. J. DiRita

§ Present address: Department of Microbiology and Immunology, University of Louisville Health Sciences Center, School of Medicine, Louisville, KY 40292.

{dagger} This paper is dedicated to the memory of Donald C. Cox, who was a great teacher, mentor, and colleague.

Infection and Immunity, May 2002, p. 2694-2699, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2694-2699.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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