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Infection and Immunity, June 2002, p. 2862-2868, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.2862-2868.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulatory Role of Interleukin-10 in Experimental Group B Streptococcal Arthritis

Manuela Puliti,1 Christina von Hunolstein,2 Claudie Verwaerde,3 Francesco Bistoni,1 Graziella Orefici,2 and Luciana Tissi1*

Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia,1 Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanitè, Rome, Italy,2 UMR-Centre National de la Recherche Scientifique, 8527 Institut Pasteur, Lille, France3

Received 15 November 2001/ Returned for modification 14 January 2002/ Accepted 1 March 2002

Intravenous inoculation of CD-1 mice with 107 CFU of type IV group B Streptococcus (GBS) results in a high incidence of diffuse septic arthritis , associated with high levels of systemic and local production of interleukin-1ß (IL-1ß) and IL-6. In this study, the role of the anti-inflammatory cytokine IL-10 in the evolution of GBS systemic infection and arthritis was evaluated. IL-10 production was evident in sera and joints of GBS-infected mice. Neutralization of endogenous IL-10 by administration of anti-IL-10 antibodies (1 mg/mouse) at the time of infection resulted in worsening of articular lesions and 60% mortality associated with early sustained production of IL-6, IL-1ß, and tumor necrosis factor alpha (TNF-{alpha}). The effect of IL-10 supplementation was assessed by administering IL-10 (100, 200, or 400 ng/mouse) once a day for 5 days, starting 1 h after infection. Treatment with IL-10 had a beneficial effect on GBS arthritis, and there was a clear-cut dose dependence. The decrease in pathology was associated with a significant reduction in IL-6, IL-1ß, and TNF-{alpha} production. Histological findings showed limited periarticular inflammation and a few-cell influx in the articular cavity of IL-10-treated mice, confirming clinical observations. In conclusion, this study provides further information concerning the role of IL-10 in regulating the immune response and inflammation and calls attention to the potential therapeutic use of IL-10 in GBS arthritis.

* Corresponding author. Mailing address: Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy. Phone: 39-075-585-7409. Fax: 39-075-585-7403. E-mail: tissi{at}unipg.it.

Editor: V. J. DiRita

Infection and Immunity, June 2002, p. 2862-2868, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.2862-2868.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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