This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sperandio, V.
Right arrow Articles by Kaper, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sperandio, V.
Right arrow Articles by Kaper, J. B.

 Previous Article  |  Next Article 

Infection and Immunity, June 2002, p. 3085-3093, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3085-3093.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quorum-Sensing Escherichia coli Regulator A: a Regulator of the LysR Family Involved in the Regulation of the Locus of Enterocyte Effacement Pathogenicity Island in Enterohemorrhagic E. coli

Vanessa Sperandio,,{dagger} Caiyi C. Li, and James B. Kaper*

Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201

Received 3 August 2001/ Returned for modification 31 October 2001/ Accepted 1 March 2002

The locus of enterocyte effacement (LEE) is a chromosomal pathogenicity island that encodes the proteins involved in the formation of the attaching and effacing lesions by enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). The LEE comprises 41 open reading frames organized in five major operons, LEE1, LEE2, LEE3, tir (LEE5), and LEE4, which encode a type III secretion system, the intimin adhesin, the translocated intimin receptor (Tir), and other effector proteins. The first gene of LEE1 encodes the Ler regulator, which activates all the other genes within the LEE. We previously reported that the LEE genes were activated by quorum sensing through Ler (V. Sperandio, J. L. Mellies, W. Nguyen, S. Shin, and J. B. Kaper, Proc. Natl. Acad. Sci. USA 96:15196-15201, 1999). In this study we report that a putative regulator in the E. coli genome is itself activated by quorum sensing. This regulator is encoded by open reading frame b3243; belongs to the LysR family of regulators; is present in EHEC, EPEC, and E. coli K-12; and shares homology with the AphB and PtxR regulators of Vibrio cholerae and Pseudomonas aeruginosa, respectively. We confirmed the activation of b3243 by quorum sensing by using transcriptional fusions and renamed this regulator quorum-sensing E. coli regulator A (QseA). We observed that QseA activated transcription of ler and therefore of the other LEE genes. An EHEC qseA mutant had a striking reduction of type III secretion activity, which was complemented when qseA was provided in trans. Similar results were also observed with a qseA mutant of EPEC. The QseA regulator is part of the regulatory cascade that regulates EHEC and EPEC virulence genes by quorum sensing.

* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Room 480, Baltimore, MD 21201. Phone: (410) 706-3004. Fax: (410) 706-0182. E-mail: jkaper{at}umaryland.edu.

Editor: V. J. DiRita

{dagger} Present address: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Tex.

Infection and Immunity, June 2002, p. 3085-3093, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3085-3093.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bhatt, S., Edwards, A. N., Nguyen, H. T. T., Merlin, D., Romeo, T., Kalman, D. (2009). The RNA Binding Protein CsrA Is a Pleiotropic Regulator of the Locus of Enterocyte Effacement Pathogenicity Island of Enteropathogenic Escherichia coli. Infect. Immun. 77: 3552-3568 [Abstract] [Full Text]  
  • House, B., Kus, J. V., Prayitno, N., Mair, R., Que, L., Chingcuanco, F., Gannon, V., Cvitkovitch, D. G., Barnett Foster, D. (2009). Acid-stress-induced changes in enterohaemorrhagic Escherichia coli O157 : H7 virulence. Microbiology 155: 2907-2918 [Abstract] [Full Text]  
  • Reading, N. C., Rasko, D. A., Torres, A. G., Sperandio, V. (2009). The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis. Proc. Natl. Acad. Sci. USA 106: 5889-5894 [Abstract] [Full Text]  
  • Price, N. L., Raivio, T. L. (2009). Characterization of the Cpx Regulon in Escherichia coli Strain MC4100. J. Bacteriol. 191: 1798-1815 [Abstract] [Full Text]  
  • Maddocks, S. E., Oyston, P. C. F. (2008). Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Microbiology 154: 3609-3623 [Abstract] [Full Text]  
  • Torres, A. G., Slater, T. M., Patel, S. D., Popov, V. L., Arenas-Hernandez, M. M. P. (2008). Contribution of the Ler- and H-NS-Regulated Long Polar Fimbriae of Escherichia coli O157:H7 during Binding to Tissue-Cultured Cells. Infect. Immun. 76: 5062-5071 [Abstract] [Full Text]  
  • Saitoh, T., Iyoda, S., Yamamoto, S., Lu, Y., Shimuta, K., Ohnishi, M., Terajima, J., Watanabe, H. (2008). Transcription of the ehx Enterohemolysin Gene Is Positively Regulated by GrlA, a Global Regulator Encoded within the Locus of Enterocyte Effacement in Enterohemorrhagic Escherichia coli. J. Bacteriol. 190: 4822-4830 [Abstract] [Full Text]  
  • Ryan, R. P., Dow, J. M. (2008). Diffusible signals and interspecies communication in bacteria. Microbiology 154: 1845-1858 [Abstract] [Full Text]  
  • Choi, J., Shin, D., Ryu, S. (2007). Implication of Quorum Sensing in Salmonella enterica Serovar Typhimurium Virulence: the luxS Gene Is Necessary for Expression of Genes in Pathogenicity Island 1. Infect. Immun. 75: 4885-4890 [Abstract] [Full Text]  
  • Kendall, M. M., Rasko, D. A., Sperandio, V. (2007). Global Effects of the Cell-to-Cell Signaling Molecules Autoinducer-2, Autoinducer-3, and Epinephrine in a luxS Mutant of Enterohemorrhagic Escherichia coli. Infect. Immun. 75: 4875-4884 [Abstract] [Full Text]  
  • Mellies, J. L., Barron, A. M. S., Carmona, A. M. (2007). Enteropathogenic and Enterohemorrhagic Escherichia coli Virulence Gene Regulation. Infect. Immun. 75: 4199-4210 [Full Text]  
  • Bansal, T., Englert, D., Lee, J., Hegde, M., Wood, T. K., Jayaraman, A. (2007). Differential Effects of Epinephrine, Norepinephrine, and Indole on Escherichia coli O157:H7 Chemotaxis, Colonization, and Gene Expression. Infect. Immun. 75: 4597-4607 [Abstract] [Full Text]  
  • Shao, H., Lamont, R. J., Demuth, D. R. (2007). Autoinducer 2 Is Required for Biofilm Growth of Aggregatibacter (Actinobacillus) actinomycetemcomitans. Infect. Immun. 75: 4211-4218 [Abstract] [Full Text]  
  • Torres, A. G., Lopez-Sanchez, G. N., Milflores-Flores, L., Patel, S. D., Rojas-Lopez, M., Martinez de la Pena, C. F., Arenas-Hernandez, M. M. P., Martinez-Laguna, Y. (2007). Ler and H-NS, Regulators Controlling Expression of the Long Polar Fimbriae of Escherichia coli O157:H7. J. Bacteriol. 189: 5916-5928 [Abstract] [Full Text]  
  • Russell, R. M., Sharp, F. C., Rasko, D. A., Sperandio, V. (2007). QseA and GrlR/GrlA Regulation of the Locus of Enterocyte Effacement Genes in Enterohemorrhagic Escherichia coli. J. Bacteriol. 189: 5387-5392 [Abstract] [Full Text]  
  • Sharp, F. C., Sperandio, V. (2007). QseA Directly Activates Transcription of LEE1 in Enterohemorrhagic Escherichia coli. Infect. Immun. 75: 2432-2440 [Abstract] [Full Text]  
  • Reading, N. C., Torres, A. G., Kendall, M. M., Hughes, D. T., Yamamoto, K., Sperandio, V. (2007). A Novel Two-Component Signaling System That Activates Transcription of an Enterohemorrhagic Escherichia coli Effector Involved in Remodeling of Host Actin. J. Bacteriol. 189: 2468-2476 [Abstract] [Full Text]  
  • Hancock, V., Klemm, P. (2007). Global Gene Expression Profiling of Asymptomatic Bacteriuria Escherichia coli during Biofilm Growth in Human Urine. Infect. Immun. 75: 966-976 [Abstract] [Full Text]  
  • Walters, M., Sperandio, V. (2006). Autoinducer 3 and Epinephrine Signaling in the Kinetics of Locus of Enterocyte Effacement Gene Expression in Enterohemorrhagic Escherichia coli.. Infect. Immun. 74: 5445-5455 [Abstract] [Full Text]  
  • Iyoda, S., Koizumi, N., Satou, H., Lu, Y., Saitoh, T., Ohnishi, M., Watanabe, H. (2006). The GrlR-GrlA Regulatory System Coordinately Controls the Expression of Flagellar and LEE-Encoded Type III Protein Secretion Systems in Enterohemorrhagic Escherichia coli.. J. Bacteriol. 188: 5682-5692 [Abstract] [Full Text]  
  • Moreira, C. G., Palmer, K., Whiteley, M., Sircili, M. P., Trabulsi, L. R., Castro, A. F. P., Sperandio, V. (2006). Bundle-Forming Pili and EspA Are Involved in Biofilm Formation by Enteropathogenic Escherichia coli. J. Bacteriol. 188: 3952-3961 [Abstract] [Full Text]  
  • Parveen, N., Cornell, K. A., Bono, J. L., Chamberland, C., Rosa, P., Leong, J. M. (2006). Bgp, a Secreted Glycosaminoglycan-Binding Protein of Borrelia burgdorferi Strain N40, Displays Nucleosidase Activity and Is Not Essential for Infection of Immunodeficient Mice.. Infect. Immun. 74: 3016-3020 [Abstract] [Full Text]  
  • Lievin-Le Moal, V., Servin, A. L. (2006). The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota. Clin. Microbiol. Rev. 19: 315-337 [Abstract] [Full Text]  
  • Iyoda, S., Watanabe, H. (2005). ClpXP Protease Controls Expression of the Type III Protein Secretion System through Regulation of RpoS and GrlR Levels in Enterohemorrhagic Escherichia coli. J. Bacteriol. 187: 4086-4094 [Abstract] [Full Text]  
  • Kaper, J. B., Sperandio, V. (2005). Bacterial Cell-to-Cell Signaling in the Gastrointestinal Tract. Infect. Immun. 73: 3197-3209 [Full Text]  
  • Clarke, M. B., Sperandio, V. (2005). Events at the Host-Microbial Interface of the Gastrointestinal Tract III. Cell-to-cell signaling among microbial flora, host, and pathogens: there is a whole lot of talking going on. Am. J. Physiol. Gastrointest. Liver Physiol. 288: G1105-G1109 [Abstract] [Full Text]  
  • John, M., Kudva, I. T., Griffin, R. W., Dodson, A. W., McManus, B., Krastins, B., Sarracino, D., Progulske-Fox, A., Hillman, J. D., Handfield, M., Tarr, P. I., Calderwood, S. B. (2005). Use of In Vivo-Induced Antigen Technology for Identification of Escherichia coli O157:H7 Proteins Expressed during Human Infection. Infect. Immun. 73: 2665-2679 [Abstract] [Full Text]  
  • Servin, A. L. (2005). Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli. Clin. Microbiol. Rev. 18: 264-292 [Abstract] [Full Text]  
  • Zhang, L., Chaudhuri, R. R., Constantinidou, C., Hobman, J. L., Patel, M. D., Jones, A. C., Sarti, D., Roe, A. J., Vlisidou, I., Shaw, R. K., Falciani, F., Stevens, M. P., Gally, D. L., Knutton, S., Frankel, G., Penn, C. W., Pallen, M. J. (2004). Regulators Encoded in the Escherichia coli Type III Secretion System 2 Gene Cluster Influence Expression of Genes within the Locus for Enterocyte Effacement in Enterohemorrhagic E. coli O157:H7. Infect. Immun. 72: 7282-7293 [Abstract] [Full Text]  
  • Van Dyk, T. K., Templeton, L. J., Cantera, K. A., Sharpe, P. L., Sariaslani, F. S. (2004). Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve?. J. Bacteriol. 186: 7196-7204 [Abstract] [Full Text]  
  • Sharma, V. K., Zuerner, R. L. (2004). Role of hha and ler in Transcriptional Regulation of the esp Operon of Enterohemorrhagic Escherichia coli O157:H7. J. Bacteriol. 186: 7290-7301 [Abstract] [Full Text]  
  • Iyoda, S., Watanabe, H. (2004). Positive effects of multiple pch genes on expression of the locus of enterocyte effacement genes and adherence of enterohaemorrhagic Escherichia coli O157 : H7 to HEp-2 cells. Microbiology 150: 2357-2571 [Abstract] [Full Text]  
  • Ren, D., Bedzyk, L. A., Ye, R. W., Thomas, S. M., Wood, T. K. (2004). Stationary-Phase Quorum-Sensing Signals Affect Autoinducer-2 and Gene Expression in Escherichia coli. Appl. Environ. Microbiol. 70: 2038-2043 [Abstract] [Full Text]  
  • Asahara, T., Shimizu, K., Nomoto, K., Hamabata, T., Ozawa, A., Takeda, Y. (2004). Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7. Infect. Immun. 72: 2240-2247 [Abstract] [Full Text]  
  • Sircili, M. P., Walters, M., Trabulsi, L. R., Sperandio, V. (2004). Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing. Infect. Immun. 72: 2329-2337 [Abstract] [Full Text]  
  • Hogardt, M., Roeder, M., Schreff, A. M., Eberl, L., Heesemann, J. (2004). Expression of Pseudomonas aeruginosa exoS is controlled by quorum sensing and RpoS. Microbiology 150: 843-851 [Abstract] [Full Text]  
  • Roe, A. J., Yull, H., Naylor, S. W., Woodward, M. J., Smith, D. G. E., Gally, D. L. (2003). Heterogeneous Surface Expression of EspA Translocon Filaments by Escherichia coli O157:H7 Is Controlled at the Posttranscriptional Level. Infect. Immun. 71: 5900-5909 [Abstract] [Full Text]  
  • Sperandio, V., Torres, A. G., Jarvis, B., Nataro, J. P., Kaper, J. B. (2003). Bacteria-host communication: The language of hormones. Proc. Natl. Acad. Sci. USA 100: 8951-8956 [Abstract] [Full Text]  
  • Clarke, S. C., Haigh, R. D., Freestone, P. P. E., Williams, P. H. (2003). Virulence of Enteropathogenic Escherichia coli, a Global Pathogen. Clin. Microbiol. Rev. 16: 365-378 [Abstract] [Full Text]  
  • Yona-Nadler, C., Umanski, T., Aizawa, S.-I., Friedberg, D., Rosenshine, I. (2003). Integration host factor (IHF) mediates repression of flagella in enteropathogenic and enterohaemorrhagic Escherichia coli. Microbiology 149: 877-884 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»