This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liang, F. T.
Right arrow Articles by Fikrig, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liang, F. T.
Right arrow Articles by Fikrig, E.

 Previous Article  |  Next Article 

Infection and Immunity, June 2002, p. 3300-3303, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3300-3303.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes

Fang Ting Liang,1 F. Kenneth Nelson,2 and Erol Fikrig1*

Section of Rheumatology, Department of Internal Medicine, School of Medicine,1 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-80312

Received 5 December 2001/ Returned for modification 19 February 2002/ Accepted 14 March 2002

A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.

* Corresponding author. Mailing address: Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, 604 LCI, 333 Cedar St., New Haven, CT 06520-8031. Phone: (203) 785-2453. Fax: (203) 785-7053. E-mail: erol.fikrig{at}yale.edu.

Editor: D. L. Burns

Infection and Immunity, June 2002, p. 3300-3303, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3300-3303.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brissette, C. A., Bykowski, T., Cooley, A. E., Bowman, A., Stevenson, B. (2009). Borrelia burgdorferi RevA Antigen Binds Host Fibronectin. Infect. Immun. 77: 2802-2812 [Abstract] [Full Text]  
  • Barbour, A. G., Jasinskas, A., Kayala, M. A., Davies, D. H., Steere, A. C., Baldi, P., Felgner, P. L. (2008). A Genome-Wide Proteome Array Reveals a Limited Set of Immunogens in Natural Infections of Humans and White-Footed Mice with Borrelia burgdorferi. Infect. Immun. 76: 3374-3389 [Abstract] [Full Text]  
  • Hughes, J. L., Nolder, C. L., Nowalk, A. J., Clifton, D. R., Howison, R. R., Schmit, V. L., Gilmore, R. D. Jr., Carroll, J. A. (2008). Borrelia burgdorferi Surface-Localized Proteins Expressed during Persistent Murine Infection Are Conserved among Diverse Borrelia spp.. Infect. Immun. 76: 2498-2511 [Abstract] [Full Text]  
  • Gautam, A., Hathaway, M., McClain, N., Ramesh, G., Ramamoorthy, R. (2008). Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter. Microbiology 154: 275-285 [Abstract] [Full Text]  
  • Nowalk, A. J., Gilmore, R. D. Jr, Carroll, J. A. (2006). Serologic Proteome Analysis of Borrelia burgdorferi Membrane-Associated Proteins. Infect. Immun. 74: 3864-3873 [Abstract] [Full Text]  
  • Brooks, C. S., Vuppala, S. R., Jett, A. M., Akins, D. R. (2006). Identification of Borrelia burgdorferi Outer Surface Proteins. Infect. Immun. 74: 296-304 [Abstract] [Full Text]  
  • Xu, Q., Seemanapalli, S. V., Lomax, L., McShan, K., Li, X., Fikrig, E., Liang, F. T. (2005). Association of Linear Plasmid 28-1 with an Arthritic Phenotype of Borrelia burgdorferi. Infect. Immun. 73: 7208-7215 [Abstract] [Full Text]  
  • Hodzic, E., Tunev, S., Feng, S., Freet, K. J., Barthold, S. W. (2005). Immunoglobulin-Regulated Expression of Borrelia burgdorferi Outer Surface Protein A In Vivo. Infect. Immun. 73: 3313-3321 [Abstract] [Full Text]  
  • Seshu, J., Boylan, J. A., Gherardini, F. C., Skare, J. T. (2004). Dissolved Oxygen Levels Alter Gene Expression and Antigen Profiles in Borrelia burgdorferi. Infect. Immun. 72: 1580-1586 [Abstract] [Full Text]  
  • Narasimhan, S., Caimano, M. J., Liang, F. T., Santiago, F., Laskowski, M., Philipp, M. T., Pachner, A. R., Radolf, J. D., Fikrig, E. (2003). Borrelia burgdorferi transcriptome in the central nervous system of non-human primates. Proc. Natl. Acad. Sci. USA 100: 15953-15958 [Abstract] [Full Text]  
  • Yang, X. F., Alani, S. M., Norgard, M. V. (2003). The response regulator Rrp2 is essential for the expression of major membrane lipoproteins in Borrelia burgdorferi. Proc. Natl. Acad. Sci. USA 100: 11001-11006 [Abstract] [Full Text]  
  • Yang, X. F., Hubner, A., Popova, T. G., Hagman, K. E., Norgard, M. V. (2003). Regulation of Expression of the Paralogous Mlp Family in Borrelia burgdorferi. Infect. Immun. 71: 5012-5020 [Abstract] [Full Text]  
  • Crother, T. R., Champion, C. I., Wu, X.-Y., Blanco, D. R., Miller, J. N., Lovett, M. A. (2003). Antigenic Composition of Borrelia burgdorferi during Infection of SCID Mice. Infect. Immun. 71: 3419-3428 [Abstract] [Full Text]  
  • Miller, J. C., Stevenson, B. (2003). Immunological and genetic characterization of Borrelia burgdorferi BapA and EppA proteins. Microbiology 149: 1113-1125 [Abstract] [Full Text]  
  • Liang, F. T., Nelson, F. K., Fikrig, E. (2002). Molecular Adaptation of Borrelia burgdorferi in the Murine Host. JEM 196: 275-280 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»