This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tabatabaei, M. Articles by Coote, J. Search for Related Content PubMed PubMed Citation Articles by Tabatabaei, M. Articles by Coote, J.

 Previous Article  |  Next Article 

Infection and Immunity, July 2002, p. 3355-3362, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3355-3362.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Protective Immunity Conferred by Attenuated aroA Derivatives of Pasteurella multocida B:2 Strains in a Mouse Model of Hemorrhagic Septicemia

Mohammad Tabatabaei,^, Zhiqi Liu,^, Anna Finucane, Roger Parton, and John Coote^*

Infection and Immunity Division, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom

Received 13 August 2001/ Returned for modification 14 September 2001/ Accepted 2 April 2002

Hemorrhagic septicemia (HS) is a fatal systemic disease of cattle and buffaloes. In South Asia HS is caused by infection with Pasteurella multocida serotype B:2. Some control is achieved with alum-precipitated or oil-adjuvanted killed whole-cell vaccines injected subcutaneously, but these vaccines provide only short-term immunity and require annual administration for effective use. Live attenuated vaccines have the advantage of a natural route of entry into the host, but for live strains to be used as vaccines, the mode of attenuation should be well defined. We constructed aroA attenuated derivatives of two P. multocida serotype B:2 strains by allelic exchange of the native aroA sequence with aroA sequences disrupted with a kanamycin resistance cassette or with marker-free aroA sequences containing an internal deletion. These strains were confirmed to be aroA mutants by PCR and Southern blot analysis, enzyme assay, and lack of growth on minimal medium. The aroA derivatives were highly attenuated for virulence in a mouse model of HS. Mouse challenge experiments showed that intraperitoneal or intranasal vaccination of an aroA strain completely protected mice against challenge with a high dose (>1,000 50% lethal doses) of either the parent strain or the other wild-type B:2 strain. The spread of the parent and the aroA derivatives to different organs was compared when the organisms were inoculated by different routes.

---

* Corresponding author. Mailing address: Infection and Immunity Division, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Phone: 44 (0) 141 330 5845. Fax: 44 (0) 141 330 4600. E-mail: j.coote{at}bio.gla.ac.uk.

Editor: R. N. Moore

Present address: Faculty of Veterinary Medicine, Zabol University, Zabol, Iran.

Present address: Center for Integrated Plant Systems, Michigan State University, East Lansing, MI 48824.

---

Infection and Immunity, July 2002, p. 3355-3362, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3355-3362.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Campoy, S., Aranda, J., Alvarez, G., Barbe, J., Llagostera, M. (2006). Isolation and Sequencing of a Temperate Transducing Phage for Pasteurella multocida.. Appl. Environ. Microbiol. 72: 3154-3160 [Abstract] [Full Text]  
• Hodgson, J. C., Finucane, A., Dagleish, M. P., Ataei, S., Parton, R., Coote, J. G. (2005). Efficacy of Vaccination of Calves against Hemorrhagic Septicemia with a Live aroA Derivative of Pasteurella multocida B:2 by Two Different Routes of Administration. Infect. Immun. 73: 1475-1481 [Abstract] [Full Text]