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Infection and Immunity, July 2002, p. 3969-3972, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3969-3972.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Cloning of a Vaccine Antigen against Infection with the Larval Stage of Echinococcus multilocularis

Charles Gauci,1* Michael Merli,2 Volker Muller,2 Conan Chow,1 Kinpei Yagi,3 Ute Mackenstedt,2 and Marshall W. Lightowlers1

Molecular Parasitology Laboratory, Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia,1 Department of Parasitology, University of Hohenheim, Stuttgart, Germany,2 Biotechnology Division, Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan3

Received 3 December 2001/ Returned for modification 25 February 2002/ Accepted 19 March 2002

Alveolar and cystic hydatidosis are caused by infection with the larval stages of Echinococcus multilocularis and Echinococcus granulosus, respectively. A host-protective antigen has been identified in E. granulosus. Here we identify the presence of a closely related protein in E. multilocularis, characterize and express a cDNA encoding the antigen (designated EM95), determine the structure of the em95 gene, and demonstrate that the EM95 recombinant protein can be used to induce significant levels of protection against challenge infection with E. multilocularis eggs in mice.

* Corresponding author. Mailing address: Molecular Parasitology Laboratory, Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, 3030 Victoria, Australia. Phone: 61 3 97312291. Fax: 61 3 97312366. E-mail: charlesg{at}unimelb.edu.au.

Editor: W. A. Petri, Jr.

Infection and Immunity, July 2002, p. 3969-3972, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3969-3972.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shi, Z., Wang, Y., Li, Z., Li, Z., Bo, Y., Ma, R., Zhao, W. (2009). Cloning, expression, and protective immunity in mice of a gene encoding the diagnostic antigen P-29 of Echinococcus granulosus. Acta Biochim Biophys Sin 41: 79-85 [Abstract] [Full Text]  
  • Zhang, W., Li, J., McManus, D. P. (2003). Concepts in Immunology and Diagnosis of Hydatid Disease. Clin. Microbiol. Rev. 16: 18-36 [Abstract] [Full Text]  


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