This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herron, L. L.
Right arrow Articles by Kapur, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herron, L. L.
Right arrow Articles by Kapur, V.

 Previous Article  |  Next Article 

Infection and Immunity, July 2002, p. 3978-3981, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3978-3981.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genome Sequence Survey Identifies Unique Sequences and Key Virulence Genes with Unusual Rates of Amino Acid Substitution in Bovine Staphylococcus aureus

Lisa L. Herron,1 Rajit Chakravarty,1,{dagger} Christopher Dwan,2 J. Ross Fitzgerald,3 James M. Musser,3 Ernest Retzel,2 and Vivek Kapur1*

Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota 55108,1 Computational Biology Center, University of Minnesota, Minneapolis, Minnesota 55455,2 Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, Montana 598403

Received 28 March 2002/ Accepted 3 April 2002

Staphylococcus aureus is a major cause of mastitis in bovine and other ruminant species. We here present the results of a comparative genomic analysis between a bovine mastitis-associated clone, RF122, and the recently sequenced human-associated clones, Mu50 and N315, of Staphylococcus aureus. A shotgun sequence survey of ~10% of the RF122 genome identified numerous unique sequences and those with elevated rates of nonsynonymous substitution. Taken together, these analyses show that there are notable differences in the genomes of bovine mastitis-associated and human clones of S. aureus and provide a framework for the identification of specific factors associated with host specificity in this major human and animal pathogen.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, University of Minnesota, 205 Veterinary Science, 1971 Commonwealth Ave., St. Paul, MN 55108. Phone: (612) 625-7712. Fax: (612) 625-5203. E-mail: vkapur{at}umn.edu.

Editor: V. J. DiRita

{dagger} Present address: Biomedical Engineering Center, University of Wisconsin, Madison, WI 53706.

Infection and Immunity, July 2002, p. 3978-3981, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3978-3981.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, D.-S., Burd, H., Liu, J., Almaas, E., Wiest, O., Barabasi, A.-L., Oltvai, Z. N., Kapatral, V. (2009). Comparative Genome-Scale Metabolic Reconstruction and Flux Balance Analysis of Multiple Staphylococcus aureus Genomes Identify Novel Antimicrobial Drug Targets. J. Bacteriol. 191: 4015-4024 [Abstract] [Full Text]  
  • Baba, T., Bae, T., Schneewind, O., Takeuchi, F., Hiramatsu, K. (2008). Genome Sequence of Staphylococcus aureus Strain Newman and Comparative Analysis of Staphylococcal Genomes: Polymorphism and Evolution of Two Major Pathogenicity Islands. J. Bacteriol. 190: 300-310 [Abstract] [Full Text]  
  • Tuchscherr, L. P. N., Gomez, M. I., Buzzola, F. R., Calvinho, L. F., Lee, J. C., Sordelli, D. O. (2007). Characterization of a New Variant of IS257 That Has Displaced the Capsule Genes within Bovine Isolates of Staphylococcus aureus. Infect. Immun. 75: 5483-5488 [Abstract] [Full Text]  
  • Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Dowson, C. G. (2005). Multilocus Sequence Typing of Staphylococcus aureus Isolated from High-Somatic-Cell-Count Cows and the Environment of an Organic Dairy Farm in the United Kingdom. J. Clin. Microbiol. 43: 4731-4736 [Abstract] [Full Text]  
  • Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Fox, L. K., Schukken, Y. H., Kruze, J. V., Bradley, A. J., Zadoks, R. N., Dowson, C. G. (2005). Multilocus Sequence Typing of Intercontinental Bovine Staphylococcus aureus Isolates. J. Clin. Microbiol. 43: 4737-4743 [Abstract] [Full Text]  
  • van Leeuwen, W. B., Melles, D. C., Alaidan, A., Al-Ahdal, M., Boelens, H. A. M., Snijders, S. V., Wertheim, H., van Duijkeren, E., Peeters, J. K., van der Spek, P. J., Gorkink, R., Simons, G., Verbrugh, H. A., van Belkum, A. (2005). Host- and Tissue-Specific Pathogenic Traits of Staphylococcus aureus. J. Bacteriol. 187: 4584-4591 [Abstract] [Full Text]  
  • Tormo, M. A., Knecht, E., Gotz, F., Lasa, I., Penades, J. R. (2005). Bap-dependent biofilm formation by pathogenic species of Staphylococcus: evidence of horizontal gene transfer?. Microbiology 151: 2465-2475 [Abstract] [Full Text]  
  • Cucarella, C., Tormo, M. A., Ubeda, C., Trotonda, M. P., Monzon, M., Peris, C., Amorena, B., Lasa, I., Penades, J. R. (2004). Role of Biofilm-Associated Protein Bap in the Pathogenesis of Bovine Staphylococcus aureus. Infect. Immun. 72: 2177-2185 [Abstract] [Full Text]  
  • Gilot, P., van Leeuwen, W. (2004). Comparative Analysis of agr Locus Diversification and Overall Genetic Variability among Bovine and Human Staphylococcus aureus Isolates. J. Clin. Microbiol. 42: 1265-1269 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»