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Infection and Immunity, August 2002, p. 4106-4111, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4106-4111.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel 33-Kilodalton Lipoprotein from Mycobacterium leprae

Yumi Maeda,1,2* Masahiko Makino,1 Dean C. Crick,2 Sebabrata Mahapatra,2 Sopa Srisungnam,1 Takemasa Takii,3 Yoshiko Kashiwabara,1 and Patrick J. Brennan2

Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama, Tokyo 189-0002,1 Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan,3 Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-16772

Received 8 March 2002/ Returned for modification 4 April 2002/ Accepted 1 May 2002

A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipoprotein was a membrane protein of M. leprae. The purified lipoprotein was found to induce production of interleukin-12 in human peripheral blood monocytes. The studies imply that M. leprae LpK is involved in protective immunity against leprosy and may be a candidate for vaccine design.

* Corresponding author. Mailing address: Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama, Tokyo 189-0002, Japan. Phone: 81-42-391-8211. Fax: 81-42-391-8212. E-mail: yumi{at}nih.go.jp.

Editor: D. L. Burns

Infection and Immunity, August 2002, p. 4106-4111, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4106-4111.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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