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Infection and Immunity, September 2002, p. 4826-4832, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.4826-4832.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

Yao-Hui Sun,1 Andreas B. den Hartigh,1 Renato de Lima Santos,2 L. Garry Adams,3 and Renée M. Tsolis1*

Department of Medical Microbiology and Immunology, Texas A&M University System Health Science Center,1 Veterinary Pathobiology, Texas A&M University, College Station, Texas,3 Universidade Federal de Minas Gerais, Belo Horizonte, Brazil2

Received 19 April 2002/ Returned for modification 28 May 2002/ Accepted 6 June 2002

The Brucella abortus virB locus is required for establishing chronic infection in the mouse. Using in vitro and in vivo models, we investigated whether virB is involved in evasion of the bactericidal activity of NADPH oxidase and the inducible nitric oxide synthase (iNOS) in macrophages. Elimination of NADPH oxidase or iNOS activity in macrophages in vitro increased recovery of wild-type B. abortus but not recovery of a virB mutant. In mice lacking either NADPH oxidase or iNOS, however, B. abortus infected and persisted to the same extent as it did in congenic C57BL/6 mice up until 60 days postinfection, suggesting that these host defense mechanisms are not critical for limiting bacterial growth in the mouse. A virB mutant did not exhibit increased survival in either of the knockout mouse strains, indicating that this locus does not contribute to evasion of nitrosative or oxidative killing mechanisms in vivo.

* Corresponding author. Mailing address: Department of Medical Microbiology, 407 Reynolds Medical Building, College Station, TX 77843-1114. Phone: (979) 845-1314. Fax: (979) 845-3479. E-mail: rtsolis{at}medicine.tamu.edu.

Editor: D. L. Burns

Infection and Immunity, September 2002, p. 4826-4832, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.4826-4832.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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