This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mawas, F.
Right arrow Articles by Vliegenthart, J. F. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mawas, F.
Right arrow Articles by Vliegenthart, J. F. G.

 Previous Article  |  Next Article 

Infection and Immunity, September 2002, p. 5107-5114, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5107-5114.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immunogenicity in a Mouse Model of a Conjugate Vaccine Made with a Synthetic Single Repeating Unit of Type 14 Pneumococcal Polysaccharide Coupled to CRM197

Fatme Mawas,1* Jutta Niggemann,2,{dagger} Christopher Jones,3 Michael J. Corbel,1 Johannis P. Kamerling,2 and Johannes F. G. Vliegenthart2

Division of Bacteriology,1 Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts EN6 3QG, United Kingdom,3 Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3508 TB Utrecht, The Netherlands2

Received 16 January 2002/ Returned for modification 29 April 2002/ Accepted 29 May 2002

Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide fragment obtained by Pn14PS depolymerization were good inhibitors. CRM197 conjugates of the tetrasaccharide and an octasaccharide mimic were prepared by using either adipic acid diester or diethyl squarate linkers. The conjugate with the tetrasaccharide chains induced anti-Pn14PS antibodies when injected subcutaneously into mice, as determined by an enzyme-linked immunosorbent assay, and antibody titers increased with oligosaccharide loading. The adipic acid-linked tetrasaccharide conjugates elicited higher antibody titers than those prepared with a squarate spacer. The lower anti-Pn14PS antibody response of the octasaccharide mimic conjugate indicates the importance of the backbone galactose residue for an appropriate antibody response. The OS-CRM197 conjugate prepared from a single repeat unit of the Pn14PS is a potential vaccine candidate.

* Corresponding author. Mailing address: Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, United Kingdom. Phone: 44-1707-654753, ext. 434. Fax: 44-707-663796. E-mail: fmawas{at}nibsc.ac.uk.

Editor: E. I. Tuomanen

{dagger} Present address: Abteilung Naturstoffchemie, Gesellschaft für Biotechnologische Forschung mbH, D-38124 Braunschweig, Germany.

Infection and Immunity, September 2002, p. 5107-5114, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5107-5114.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Safari, D., Dekker, H. A. T., Joosten, J. A. F., Michalik, D., de Souza, A. C., Adamo, R., Lahmann, M., Sundgren, A., Oscarson, S., Kamerling, J. P., Snippe, H. (2008). Identification of the Smallest Structure Capable of Evoking Opsonophagocytic Antibodies against Streptococcus pneumoniae Type 14. Infect. Immun. 76: 4615-4623 [Abstract] [Full Text]  
  • Xin, H., Dziadek, S., Bundle, D. R., Cutler, J. E. (2008). Synthetic glycopeptide vaccines combining {beta}-mannan and peptide epitopes induce protection against candidiasis. Proc. Natl. Acad. Sci. USA 105: 13526-13531 [Abstract] [Full Text]  
  • Kubler-Kielb, J., Liu, T.-Y., Mocca, C., Majadly, F., Robbins, J. B., Schneerson, R. (2006). Additional Conjugation Methods and Immunogenicity of Bacillus anthracis Poly-{gamma}-D-Glutamic Acid-Protein Conjugates.. Infect. Immun. 74: 4744-4749 [Abstract] [Full Text]  
  • Phalipon, A., Costachel, C., Grandjean, C., Thuizat, A., Guerreiro, C., Tanguy, M., Nato, F., Vulliez-Le Normand, B., Belot, F., Wright, K., Marcel-Peyre, V., Sansonetti, P. J., Mulard, L. A. (2006). Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine. J. Immunol. 176: 1686-1694 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»