This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choi, B.-K.
Right arrow Articles by Yoo, Y.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, B.-K.
Right arrow Articles by Yoo, Y.-J.

 Previous Article  |  Next Article 

Infection and Immunity, January 2003, p. 226-233, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.226-233.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Induction of Osteoclastogenesis and Matrix Metalloproteinase Expression by the Lipooligosaccharide of Treponema denticola

Bong-Kyu Choi,1,2 Hyun Jung Lee,1 Jung Hwa Kang,1 Gook Jin Jeong,1 Cheon Ki Min,1 and Yun-Jung Yoo1,3*

Department of Oral Biology,1 Oral Science Research Center, College of Dentistry,3 Brain Korea 21 Project for Medical Sciences, Yonsei University, Seoul, Korea2

Received 7 June 2002/ Returned for modification 15 July 2002/ Accepted 18 September 2002

Alveolar bone destruction is a characteristic feature of periodontitis. Treponema denticola is known to be involved in periodontitis. To elucidate the role of T. denticola in alveolar bone destruction in periodontitis, the effects of lipooligosaccharide (LOS) from T. denticola on osteoclast formation and on expression of osteoclast differentiation factor (ODF) and osteoprotegerin (OPG) mRNAs were examined in a coculture system by using mouse calvaria and bone marrow cells. In addition, the effect of T. denticola LOS on expression of matrix metalloproteinases (MMPs), which are involved in bone resorption, was estimated in mouse calvaria-derived osteoblastic cells. When the mouse calvaria and bone marrow cells were challenged with LOS (0.1 to 10 µg/ml) for 4 days, the number of tartrate-resistant acid phosphatase-positive multinucleated cells increased in a dose-dependent manner. The expression of ODF mRNA increased, while OPG mRNA expression decreased. Polymyxin B changed the effect of LOS (10 µg/ml) on ODF and OPG mRNA expression to the control level. LOS (10 µg/ml) stimulated prostaglandin E2 (PGE2) production in the cocultures. Adding indomethacin, an inhibitor of prostaglandin synthesis, resulted in a reduction in the number of osteoclasts induced by LOS and eliminated the effect of T. denticola LOS on ODF and OPG mRNA expression. T. denticola LOS increased the levels of mRNAs encoding MMP-3, -8, -9, -10, -13, and -14. Expression of one of these mRNAs, MMP-9 mRNA, was significantly induced by T. denticola LOS. These findings suggest that LOS from T. denticola stimulates osteoclastogenesis and MMP expression. Up-regulation of ODF and down-regulation of OPG by a PGE2-dependent mechanism were involved in the osteoclastogenesis induced by T. denticola LOS.

* Corresponding author. Mailing address: Department of Oral Biology, College of Dentistry, Yonsei University, 134 Shinchon-Dong, Seodaemun-Gu, 120-752 Seoul, Korea. Phone: 82-02-361-8042. Fax: 82-02-364-1085. E-mail: yu618{at}yumc.yonsei.ac.kr.

Editor: J. D. Clements

Infection and Immunity, January 2003, p. 226-233, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.226-233.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jun, H.-K., Kang, Y.-M., Lee, H.-R., Lee, S.-H., Choi, B.-K. (2008). Highly Conserved Surface Proteins of Oral Spirochetes as Adhesins and Potent Inducers of Proinflammatory and Osteoclastogenic Factors. Infect. Immun. 76: 2428-2438 [Abstract] [Full Text]  
  • Foschi, F., Izard, J., Sasaki, H., Sambri, V., Prati, C., Muller, R., Stashenko, P. (2006). Treponema denticola in Disseminating Endodontic Infections. JDR 85: 761-765 [Abstract] [Full Text]  
  • Lee, S.-H., Kim, K.-K., Rhyu, I.-C., Koh, S., Lee, D.-S., Choi, B.-K. (2006). Phenol/water extract of Treponema socranskii subsp. socranskii as an antagonist of Toll-like receptor 4 signalling. Microbiology 152: 535-546 [Abstract] [Full Text]  
  • Suzuki, N., Yoshida, A., Nakano, Y. (2005). Quantitative Analysis of Multi-Species Oral Biofilms by TaqMan Real-Time PCR. Clin Med Res 3: 176-185 [Abstract] [Full Text]  
  • Lee, S.-H., Kim, K.-K., Choi, B.-K. (2005). Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum, the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections. Infect. Immun. 73: 268-276 [Abstract] [Full Text]  
  • Belibasakis, G. N., Johansson, A., Wang, Y., Chen, C., Kalfas, S., Lerner, U. H. (2005). The Cytolethal Distending Toxin Induces Receptor Activator of NF-{kappa}B Ligand Expression in Human Gingival Fibroblasts and Periodontal Ligament Cells. Infect. Immun. 73: 342-351 [Abstract] [Full Text]  
  • Nociti, F.H. Jr., Foster, B.L., Barros, S.P., Darveau, R.P., Somerman, M.J. (2004). Cementoblast Gene Expression is Regulated by Porphyromonas gingivalis Lipopolysaccharide Partially via Toll-like Receptor-4/MD-2. JDR 83: 602-607 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»