This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shreedhar, V. K.
Right arrow Articles by Neutra, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shreedhar, V. K.
Right arrow Articles by Neutra, M. R.

 Previous Article  |  Next Article 

Infection and Immunity, January 2003, p. 504-509, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.504-509.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cholera Toxin Induces Migration of Dendritic Cells from the Subepithelial Dome Region to T- and B-Cell Areas of Peyer's Patches

Vijay K. Shreedhar,1,{dagger} Brian L. Kelsall,2 and Marian R. Neutra1*

GI Cell Biology Laboratory, Children's Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115,1 Mucosal Immunity Section, Laboratory of Clinical Investigation, National Institutes of Health, Bethesda Maryland 208922

Received 18 April 2002/ Returned for modification 2 July 2002/ Accepted 27 September 2002

Intestinal M cells deliver macromolecules, particles, and pathogens into the subepithelial dome (SED) region of Peyer's patch mucosa, an area rich in dendritic cells (DCs). We tested whether uptake of the mucosal adjuvant cholera toxin (CT) or live Salmonella bacteria can induce DC migration within Peyer's patches. Virus-sized, fluorescent polystyrene microparticles were efficiently transported by M cells and ingested by CD11c+, CD11b-, and CD8a- DCs in the SED region. DCs loaded with microparticles remained in the SED for up to 14 days. CT (but not the CT B subunit) and live attenuated Salmonella enterica serovar Typhimurium bacteria induced migration of the microparticle-loaded DCs from the SED region into underlying B-cell follicles and adjacent parafollicular T-cell zones. Our data provide the first demonstration that DCs move in response to enterotoxin adjuvants and live bacteria that enter the mucosa via M cells.

* Corresponding author. Mailing address: GI Cell Biology Laboratory, Enders 1220, Children's Hospital, 300 Longwood Ave., Boston MA 02115. Phone: (617) 355-6229. Fax: (617) 264-2876. E-mail: marian.neutra{at}tch.harvard.edu.

Editor: J. D. Clements

{dagger} Present address: Viral Vaccine Immunology, Wyeth-Ayerst Pharmaceuticals, Pearl River, NY 10965.

Infection and Immunity, January 2003, p. 504-509, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.504-509.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Maldonado-Arocho, F. J., Bradley, K. A. (2009). Anthrax Edema Toxin Induces Maturation of Dendritic Cells and Enhances Chemotaxis towards Macrophage Inflammatory Protein 3{beta}. Infect. Immun. 77: 2036-2042 [Abstract] [Full Text]  
  • Blazquez, A. B., Berin, M. C. (2008). Gastrointestinal Dendritic Cells Promote Th2 Skewing via OX40L. J. Immunol. 180: 4441-4450 [Abstract] [Full Text]  
  • Wakabayashi, A., Nakagawa, Y., Shimizu, M., Moriya, K., Nishiyama, Y., Takahashi, H. (2008). Suppression of an Already Established Tumor Growing through Activated Mucosal CTLs Induced by Oral Administration of Tumor Antigen with Cholera Toxin. J. Immunol. 180: 4000-4010 [Abstract] [Full Text]  
  • Shaykhiev, R., Bals, R. (2007). Interactions between epithelial cells and leukocytes in immunity and tissue homeostasis. J. Leukoc. Biol. 82: 1-15 [Abstract] [Full Text]  
  • Halle, S., Bumann, D., Herbrand, H., Willer, Y., Dahne, S., Forster, R., Pabst, O. (2007). Solitary Intestinal Lymphoid Tissue Provides a Productive Port of Entry for Salmonella enterica Serovar Typhimurium. Infect. Immun. 75: 1577-1585 [Abstract] [Full Text]  
  • Pabst, O., Herbrand, H., Friedrichsen, M., Velaga, S., Dorsch, M., Berhardt, G., Worbs, T., Macpherson, A. J., Forster, R. (2006). Adaptation of Solitary Intestinal Lymphoid Tissue in Response to Microbiota and Chemokine Receptor CCR7 Signaling. J. Immunol. 177: 6824-6832 [Abstract] [Full Text]  
  • Kwa, S.-f., Beverley, P., Smith, A. L. (2006). Peyer's Patches Are Required for the Induction of Rapid Th1 Responses in the Gut and Mesenteric Lymph Nodes during an Enteric Infection.. J. Immunol. 176: 7533-7541 [Abstract] [Full Text]  
  • Chabot, S., Wagner, J. S., Farrant, S., Neutra, M. R. (2006). TLRs Regulate the Gatekeeping Functions of the Intestinal Follicle-Associated Epithelium. J. Immunol. 176: 4275-4283 [Abstract] [Full Text]  
  • Crane, J. K., Choudhari, S. S., Naeher, T. M., Duffey, M. E. (2006). Mutual Enhancement of Virulence by Enterotoxigenic and Enteropathogenic Escherichia coli. Infect. Immun. 74: 1505-1515 [Abstract] [Full Text]  
  • Grdic, D., Ekman, L., Schon, K., Lindgren, K., Mattsson, J., Magnusson, K.-E., Ricciardi-Castagnoli, P., Lycke, N. (2005). Splenic Marginal Zone Dendritic Cells Mediate the Cholera Toxin Adjuvant Effect: Dependence on the ADP-Ribosyltransferase Activity of the Holotoxin. J. Immunol. 175: 5192-5202 [Abstract] [Full Text]  
  • Favre, L., Spertini, F., Corthesy, B. (2005). Secretory IgA Possesses Intrinsic Modulatory Properties Stimulating Mucosal and Systemic Immune Responses. J. Immunol. 175: 2793-2800 [Abstract] [Full Text]  
  • Smith, D. W., Nagler-Anderson, C. (2005). Preventing Intolerance: The Induction of Nonresponsiveness to Dietary and Microbial Antigens in the Intestinal Mucosa. J. Immunol. 174: 3851-3857 [Abstract] [Full Text]  
  • Turnbull, E. L., Yrlid, U., Jenkins, C. D., MacPherson, G. G. (2005). Intestinal Dendritic Cell Subsets: Differential Effects of Systemic TLR4 Stimulation on Migratory Fate and Activation In Vivo. J. Immunol. 174: 1374-1384 [Abstract] [Full Text]  
  • van Wijk, F., Hoeks, S., Nierkens, S., Koppelman, S. J., van Kooten, P., Boon, L., Knippels, L. M. J., Pieters, R. (2005). CTLA-4 Signaling Regulates the Intensity of Hypersensitivity Responses to Food Antigens, but is Not Decisive in the Induction of Sensitization. J. Immunol. 174: 174-179 [Abstract] [Full Text]  
  • Fleeton, M. N., Contractor, N., Leon, F., Wetzel, J. D., Dermody, T. S., Kelsall, B. L. (2004). Peyer's Patch Dendritic Cells Process Viral Antigen from Apoptotic Epithelial Cells in the Intestine of Reovirus-infected Mice. JEM 200: 235-245 [Abstract] [Full Text]  
  • Mathers, A. R., Cuff, C. F. (2004). Role of Interleukin-4 (IL-4) and IL-10 in Serum Immunoglobulin G Antibody Responses following Mucosal or Systemic Reovirus Infection. J. Virol. 78: 3352-3360 [Abstract] [Full Text]  
  • Rey, J., Garin, N., Spertini, F., Corthesy, B. (2004). Targeting of Secretory IgA to Peyer's Patch Dendritic and T Cells after Transport by Intestinal M Cells. J. Immunol. 172: 3026-3033 [Abstract] [Full Text]  
  • Pabst, O., Ohl, L., Wendland, M., Wurbel, M.-A., Kremmer, E., Malissen, B., Forster, R. (2004). Chemokine Receptor CCR9 Contributes to the Localization of Plasma Cells to the Small Intestine. JEM 199: 411-416 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»