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Infection and Immunity, January 2003, p. 546-549, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.546-549.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

MprF-Mediated Lysinylation of Phospholipids in Staphylococcus aureus Leads to Protection against Oxygen-Independent Neutrophil Killing

Sascha A. Kristian,1,2 Manuela Dürr,1 Jos A. G. Van Strijp,3 Birgid Neumeister,2 and Andreas Peschel1*

Microbial Genetics, University of Tübingen,1 Department of Transfusion Medicine, University Hospital Tübingen, 72076 Tübingen, Germany,2 Eijkman Winkler Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands3

Received 9 May 2002/ Returned for modification 20 June 2002/ Accepted 7 October 2002

Staphylococcus aureus achieves resistance to defensins and similar cationic antimicrobial peptides (CAMPs) by modifying anionic membrane lipids via MprF with L-lysine, which leads to repulsion of these host defense molecules. S. aureus {Delta}mprF, which lacks the modification, was very efficiently killed by neutrophil defensins and CAMP-producing leukocytes, even when oxygen-dependent killing was disrupted, but was as susceptible as wild-type bacteria to inactivation by myeloperoxidase or human monocytes lacking defensins. These results demonstrate the impact and specificity of MprF-mediated CAMP resistance and underscore the role of defensin-like peptides in innate host defense.

* Corresponding author. Mailing address: Microbial Genetics, University of Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. Phone: 49-7071-297-7623. Fax: 49-7071-29-5065. E-mail: andreas.peschel{at}uni-tuebingen.de.

Editor: S. H. E. Kaufmann

Infection and Immunity, January 2003, p. 546-549, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.546-549.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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