This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, S.
Right arrow Articles by Clancy, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, S.
Right arrow Articles by Clancy, C. J.

 Previous Article  |  Next Article 

Infection and Immunity, October 2003, p. 6101-6103, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6101-6103.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native Locus

Shaoji Cheng,1 M. Hong Nguyen,1,2,3 Zongde Zhang,1 Hongyan Jia,1 Martin Handfield,4 and Cornelius J. Clancy1,2,3*

Departments of Medicine,1 Molecular Genetics and Microbiology, University of Florida College of Medicine ,3 Center for Molecular Microbiology and Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida 32610,4 North Florida/South Georgia V.A. Medical Center, Gainesville, Florida 326082

Received 1 April 2003/ Returned for modification 15 May 2003/ Accepted 9 July 2003

Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Box 100266 JHMHC, Gainesville, FL 32610. Phone: (352) 376-1611, ext. 6438. Fax: (352) 379-4015. E-mail: clancyn{at}medicine.ufl.edu.

Editor: T. R. Kozel

Infection and Immunity, October 2003, p. 6101-6103, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6101-6103.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hao, B., Clancy, C. J., Cheng, S., Raman, S. B., Iczkowski, K. A., Nguyen, M. H. (2009). Candida albicans RFX2 Encodes a DNA Binding Protein Involved in DNA Damage Responses, Morphogenesis, and Virulence. Eukaryot Cell 8: 627-639 [Abstract] [Full Text]  
  • Balish, E. (2009). A URA3 null mutant of Candida albicans (CAI-4) causes oro-oesophageal and gastric candidiasis and is lethal for gnotobiotic, transgenic mice (Tg{epsilon}26) that are deficient in both natural killer and T cells. J Med Microbiol 58: 290-295 [Abstract] [Full Text]  
  • Lermann, U., Morschhauser, J. (2008). Secreted aspartic proteases are not required for invasion of reconstituted human epithelia by Candida albicans. Microbiology 154: 3281-3295 [Abstract] [Full Text]  
  • Fu, Y., Luo, G., Spellberg, B. J., Edwards, J. E. Jr., Ibrahim, A. S. (2008). Gene Overexpression/Suppression Analysis of Candidate Virulence Factors of Candida albicans. Eukaryot Cell 7: 483-492 [Abstract] [Full Text]  
  • Ding, C., Butler, G. (2007). Development of a Gene Knockout System in Candida parapsilosis Reveals a Conserved Role for BCR1 in Biofilm Formation. Eukaryot Cell 6: 1310-1319 [Abstract] [Full Text]  
  • Jezewski, S., von der Heide, M., Poltermann, S., Hartl, A., Kunkel, W., Zipfel, P. F., Eck, R. (2007). Role of the Vps34p-interacting protein Ade5,7p in hyphal growth and virulence of Candida albicans. Microbiology 153: 2351-2362 [Abstract] [Full Text]  
  • Biswas, S., Van Dijck, P., Datta, A. (2007). Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71: 348-376 [Abstract] [Full Text]  
  • Piekarska, K., Mol, E., van den Berg, M., Hardy, G., van den Burg, J., van Roermund, C., MacCallum, D., Odds, F., Distel, B. (2006). Peroxisomal Fatty Acid {beta}-Oxidation Is Not Essential for Virulence of Candida albicans. Eukaryot Cell 5: 1847-1856 [Abstract] [Full Text]  
  • Miyazaki, T., Miyazaki, Y., Izumikawa, K., Kakeya, H., Miyakoshi, S., Bennett, J. E., Kohno, S. (2006). Fluconazole Treatment Is Effective against a Candida albicans erg3/erg3 Mutant In Vivo Despite In Vitro Resistance. Antimicrob. Agents Chemother. 50: 580-586 [Abstract] [Full Text]  
  • Barelle, C. J., Richard, M. L., Gaillardin, C., Gow, N. A. R., Brown, A. J. P. (2006). Candida albicans VAC8 Is Required for Vacuolar Inheritance and Normal Hyphal Branching. Eukaryot Cell 5: 359-367 [Abstract] [Full Text]  
  • Martinez-Lopez, R., Park, H., Myers, C. L., Gil, C., Filler, S. G. (2006). Candida albicans Ecm33p Is Important for Normal Cell Wall Architecture and Interactions with Host Cells. Eukaryot Cell 5: 140-147 [Abstract] [Full Text]  
  • Cheng, S., Clancy, C. J., Checkley, M. A., Zhang, Z., Wozniak, K. L., Seshan, K. R., Jia, H. Y., Fidel, P. Jr, Cole, G., Nguyen, M. H. (2005). The Role of Candida albicans NOT5 in Virulence Depends upon Diverse Host Factors In Vivo. Infect. Immun. 73: 7190-7197 [Abstract] [Full Text]  
  • Hromatka, B. S., Noble, S. M., Johnson, A. D. (2005). Transcriptional Response of Candida albicans to Nitric Oxide and the Role of the YHB1 Gene in Nitrosative Stress and Virulence. Mol. Biol. Cell 16: 4814-4826 [Abstract] [Full Text]  
  • Badrane, H., Cheng, S., Nguyen, M. H., Jia, H. Y., Zhang, Z., Weisner, N., Clancy, C. J. (2005). Candida albicans IRS4 contributes to hyphal formation and virulence after the initial stages of disseminated candidiasis. Microbiology 151: 2923-2931 [Abstract] [Full Text]  
  • Rouabhia, M., Schaller, M., Corbucci, C., Vecchiarelli, A., Prill, S. K.-H., Giasson, L., Ernst, J. F. (2005). Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases. Infect. Immun. 73: 4571-4580 [Abstract] [Full Text]  
  • Poltermann, S., Nguyen, M., Gunther, J., Wendland, J., Hartl, A., Kunkel, W., Zipfel, P. F., Eck, R. (2005). The putative vacuolar ATPase subunit Vma7p of Candida albicans is involved in vacuole acidification, hyphal development and virulence. Microbiology 151: 1645-1655 [Abstract] [Full Text]  
  • Sharkey, L. L., Liao, W.-l., Ghosh, A. K., Fonzi, W. A. (2005). Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans. Microbiology 151: 1061-1071 [Abstract] [Full Text]  
  • Singleton, D. R., Masuoka, J., Hazen, K. C. (2005). Surface Hydrophobicity Changes of Two Candida albicans Serotype B mnn4{Delta} Mutants. Eukaryot Cell 4: 639-648 [Abstract] [Full Text]  
  • Du, C., Calderone, R., Richert, J., Li, D. (2005). Deletion of the SSK1 Response Regulator Gene in Candida albicans Contributes to Enhanced Killing by Human Polymorphonuclear Neutrophils. Infect. Immun. 73: 865-871 [Abstract] [Full Text]  
  • Shen, J., Guo, W., Kohler, J. R. (2005). CaNAT1, a Heterologous Dominant Selectable Marker for Transformation of Candida albicans and Other Pathogenic Candida Species. Infect. Immun. 73: 1239-1242 [Abstract] [Full Text]  
  • Noble, S. M., Johnson, A. D. (2005). Strains and Strategies for Large-Scale Gene Deletion Studies of the Diploid Human Fungal Pathogen Candida albicans. Eukaryot Cell 4: 298-309 [Abstract] [Full Text]  
  • Brand, A., MacCallum, D. M., Brown, A. J. P., Gow, N. A. R., Odds, F. C. (2004). Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus. Eukaryot Cell 3: 900-909 [Abstract] [Full Text]  
  • Ullmann, B. D., Myers, H., Chiranand, W., Lazzell, A. L., Zhao, Q., Vega, L. A., Lopez-Ribot, J. L., Gardner, P. R., Gustin, M. C. (2004). Inducible Defense Mechanism against Nitric Oxide in Candida albicans. Eukaryot Cell 3: 715-723 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»