This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deng, J.
Right arrow Articles by Fishelson, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deng, J.
Right arrow Articles by Fishelson, Z.

 Previous Article  |  Next Article 

Infection and Immunity, November 2003, p. 6402-6410, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6402-6410.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin

Jiusheng Deng,1 Daniel Gold,1 Philip T. LoVerde,2 and Zvi Fishelson3*

Departments of Human Microbiology,1 Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel,3 Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo, New York2

Received 28 April 2003/ Returned for modification 18 June 2003/ Accepted 18 August 2003

Larvae and adults of the parasitic blood fluke Schistosoma mansoni are resistant to killing by human complement. An earlier search by Parizade et al. for a schistosome complement inhibitor identified a 94-kDa surface protein which was named SCIP-1 (M. Parizade, R. Arnon, P. J. Lachmann, and Z. Fishelson, J. Exp. Med. 179:1625-1636, 1994). Following partial purification and analysis by mass spectrometry, we have determined SCIP-1 to be a surface-exposed form of the muscle protein paramyosin. As shown by immunofluorescence, anti-paramyosin antibodies label the surface of live schistosomula and adult worms. Like SCIP-1, purified native paramyosin reacts with a polyclonal rabbit anti-human CD59 antiserum, as shown by Western blot analysis. Also, the human complement components C8 and C9 bind to recombinant and native paramyosin. Analysis of paramyosin binding to fragments of C9 generated by thrombin or trypsin has demonstrated that paramyosin binds to C9 at a position located between Gly245 and Arg391. Paramyosin inhibited Zn2+-induced C9 polymerization and poly-C9 deposition onto rabbit erythrocytes (ER). In addition, paramyosin inhibited lysis of ER and of sensitized sheep erythrocytes by human complement. Finally, anti-paramyosin antibodies enhanced in vitro killing of schistosomula by normal and C4-depleted human complement. Taken together, these findings suggest that an exogenous form of S. mansoni paramyosin inhibits activation of the terminal pathway of complement and thus has an important immunomodulatory role in schistosomiasis.

* Corresponding author. Mailing address: Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, P.O. Box 39040, Tel Aviv 69978, Israel. Phone: 972 3 6409620. Fax: 972 3 6407432. E-mail: lifish{at}post.tau.ac.il.

Editor: J. M. Mansfield

Infection and Immunity, November 2003, p. 6402-6410, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6402-6410.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bergstrom, F. C., Reynolds, S., Johnstone, M., Pike, R. N., Buckle, A. M., Kemp, D. J., Fischer, K., Blom, A. M. (2009). Scabies Mite Inactivated Serine Protease Paralogs Inhibit the Human Complement System. J. Immunol. 182: 7809-7817 [Abstract] [Full Text]  
  • Jiz, M., Wu, H.-W., Meng, R., Pond-Tor, S., Reynolds, M., Friedman, J. F., Olveda, R., Acosta, L., Kurtis, J. D. (2008). Pilot-Scale Production and Characterization of Paramyosin, a Vaccine Candidate for Schistosomiasis Japonica. Infect. Immun. 76: 3164-3169 [Abstract] [Full Text]  
  • Moskovich, O., Fishelson, Z. (2007). Live Cell Imaging of Outward and Inward Vesiculation Induced by the Complement C5b-9 Complex. J. Biol. Chem. 282: 29977-29986 [Abstract] [Full Text]  
  • NARA, T., IIZUMI, K., OHMAE, H., SY, O. S., TSUBOTA, S., INABA, Y., TSUBOUCHI, A., TANABE, M., KOJIMA, S., AOKI, T. (2007). ANTIBODY ISOTYPE RESPONSES TO PARAMYOSIN, A VACCINE CANDIDATE FOR SCHISTOSOMIASIS, AND THEIR CORRELATIONS WITH RESISTANCE AND FIBROSIS IN PATIENTS INFECTED WITH SCHISTOSOMA JAPONICUM IN LEYTE, THE PHILIPPINES. Am J Trop Med Hyg 76: 384-391 [Abstract] [Full Text]  
  • Braschi, S., Wilson, R. A. (2006). Proteins Exposed at the Adult Schistosome Surface Revealed by Biotinylation. Mol. Cell. Proteomics 5: 347-356 [Abstract] [Full Text]  
  • Knudsen, G. M., Medzihradszky, K. F., Lim, K.-C., Hansell, E., McKerrow, J. H. (2005). Proteomic Analysis of Schistosoma mansoni Cercarial Secretions. Mol. Cell. Proteomics 4: 1862-1875 [Abstract] [Full Text]  
  • Giacomin, P. R., Wang, H., Gordon, D. L., Botto, M., Dent, L. A. (2005). Loss of Complement Activation and Leukocyte Adherence as Nippostrongylus brasiliensis Develops within the Murine Host. Infect. Immun. 73: 7442-7449 [Abstract] [Full Text]  
  • Pilzer, D., Fishelson, Z. (2005). Mortalin/GRP75 promotes release of membrane vesicles from immune attacked cells and protection from complement-mediated lysis. Int Immunol 17: 1239-1248 [Abstract] [Full Text]  
  • Hooper, S. L., Thuma, J. B. (2005). Invertebrate Muscles: Muscle Specific Genes and Proteins. Physiol. Rev. 85: 1001-1060 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»