This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Althouse, C.
Right arrow Articles by Isaacson, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Althouse, C.
Right arrow Articles by Isaacson, R. E.

 Previous Article  |  Next Article 

Infection and Immunity, November 2003, p. 6446-6452, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6446-6452.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Type 1 Fimbriae of Salmonella enterica Serovar Typhimurium Bind to Enterocytes and Contribute to Colonization of Swine In Vivo

Carrie Althouse,1 Sheila Patterson,1 Paula Fedorka-Cray,2,{dagger} and Richard E. Isaacson1,3*

Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois 61802,1 Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota 55108,3 National Animal Disease Center, United States Department of Agriculture, Ames, Iowa 500102

Received 9 April 2003/ Returned for modification 16 June 2003/ Accepted 15 July 2003

Salmonella enterica serovar Typhimurium strain 798 is a clinical isolate from a pig and is known to be able to cause persistent, asymptomatic infections. This strain also is known to exist in two phenotypes (adhesive and nonadhesive to enterocytes) and can switch between the two phenotypes at a rate consistent with phase variation. Cells in the adhesive phenotype are more readily phagocytosed by leukocytes than nonadhesive cells. Once in a leukocyte, adhesive-phase cells survive while nonadhesive-phase cells die. In the present study, nonadhesive mutants were obtained with the transposon TnphoA. A nonadhesive mutant was selected for study and was shown by electron microscopy not to produce fimbriae. The gene encoding the adhesin was cloned and sequenced. Based on its sequence, the adhesin was shown to be FimA, the major subunit of type 1 fimbriae. The nonadhesive mutant was attenuated in its ability to colonize both mouse and pig intestines, but remained capable of systemic spread in mice. The nonadhesive mutant was phagocytosed to the same extent as parental cells in the adhesive phase and then survived intracellularly. These results demonstrated that type 1 fimbriae were important for attachment to enterocytes and promoted intestinal colonization. However, they were not important in promoting phagocytosis or intracellular survival.

* Corresponding author. Mailing address: Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Ave., St. Paul, Minnesota 55108. Phone: (612) 624-0701. Fax: (612) 625-5203. E-mail: isaac015{at}umn.edu.

Editor: A. D. O'Brien

{dagger} Present address: USDA Agricultural Research Service, Richard Russell Research Center, Athens, GA 30605.

Infection and Immunity, November 2003, p. 6446-6452, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6446-6452.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stevens, M. P., Humphrey, T. J., Maskell, D. J. (2009). Molecular insights into farm animal and zoonotic Salmonella infections. Phil Trans R Soc B 364: 2709-2723 [Abstract] [Full Text]  
  • Guo, A., Cao, S., Tu, L., Chen, P., Zhang, C., Jia, A., Yang, W., Liu, Z., Chen, H., Schifferli, D. M. (2009). FimH alleles direct preferential binding of Salmonella to distinct mammalian cells or to avian cells. Microbiology 155: 1623-1633 [Abstract] [Full Text]  
  • Boyen, F, Pasmans, F, Van Immerseel, F, Donne, E, Morgan, E, Ducatelle, R, Haesebrouck, F (2009). Porcine in vitro and in vivo models to assess the virulence of Salmonella enterica serovar Typhimurium for pigs. Lab Anim 43: 46-52 [Abstract] [Full Text]  
  • Maisey, H. C., Quach, D., Hensler, M. E., Liu, G. Y., Gallo, R. L., Nizet, V., Doran, K. S. (2008). A group B streptococcal pilus protein promotes phagocyte resistance and systemic virulence. FASEB J. 22: 1715-1724 [Abstract] [Full Text]  
  • Field, T. R., Layton, A. N., Bispham, J., Stevens, M. P., Galyov, E. E. (2008). Identification of Novel Genes and Pathways Affecting Salmonella Type III Secretion System 1 Using a Contact-Dependent Hemolysis Assay. J. Bacteriol. 190: 3393-3398 [Abstract] [Full Text]  
  • Foley, S. L., Lynne, A. M. (2008). Food animal-associated Salmonella challenges: Pathogenicity and antimicrobial resistance. J ANIM SCI 86: E173-E187 [Abstract] [Full Text]  
  • Huang, Y., Leming, C. L., Suyemoto, M., Altier, C. (2007). Genome-Wide Screen of Salmonella Genes Expressed during Infection in Pigs, Using In Vivo Expression Technology. Appl. Environ. Microbiol. 73: 7522-7530 [Abstract] [Full Text]  
  • Ou, H.-Y., Ju, C. T. S., Thong, K.-L., Ahmad, N., Deng, Z., Barer, M. R., Rajakumar, K. (2007). Translational Genomics to Develop a Salmonella enterica Serovar Paratyphi A Multiplex Polymerase Chain Reaction Assay. J. Mol. Diagn. 9: 624-630 [Abstract] [Full Text]  
  • Carnell, S. C., Bowen, A., Morgan, E., Maskell, D. J., Wallis, T. S., Stevens, M. P. (2007). Role in virulence and protective efficacy in pigs of Salmonella enterica serovar Typhimurium secreted components identified by signature-tagged mutagenesis. Microbiology 153: 1940-1952 [Abstract] [Full Text]  
  • Ledeboer, N. A., Frye, J. G., McClelland, M., Jones, B. D. (2006). Salmonella enterica Serovar Typhimurium Requires the Lpf, Pef, and Tafi Fimbriae for Biofilm Formation on HEp-2 Tissue Culture Cells and Chicken Intestinal Epithelium.. Infect. Immun. 74: 3156-3169 [Abstract] [Full Text]  
  • Weening, E. H., Barker, J. D., Laarakker, M. C., Humphries, A. D., Tsolis, R. M., Baumler, A. J. (2005). The Salmonella enterica Serotype Typhimurium lpf, bcf, stb, stc, std, and sth Fimbrial Operons Are Required for Intestinal Persistence in Mice. Infect. Immun. 73: 3358-3366 [Abstract] [Full Text]  
  • van Diemen, P. M., Dziva, F., Stevens, M. P., Wallis, T. S. (2005). Identification of Enterohemorrhagic Escherichia coli O26:H- Genes Required for Intestinal Colonization in Calves. Infect. Immun. 73: 1735-1743 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»