IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jacobi, C. A.
Right arrow Articles by Heesemann, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jacobi, C. A.
Right arrow Articles by Heesemann, J.

 Previous Article  |  Next Article 

Infection and Immunity, November 2003, p. 6624-6626, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6624-6626.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of N-(3-Oxohexanoyl)-L-Homoserine Lactone in Mice Infected with Yersinia enterocolitica Serotype O8

Christoph A. Jacobi,1* Alexandra Bach,1 Leo Eberl,2 Anette Steidle,2 and Jürgen Heesemann1

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillian University Munich, D-80336 Munich,1 Department of Microbiology, Technical University Munich, D-85350 Freising, Germany2

Received 5 May 2003/ Returned for modification 16 July 2003/ Accepted 7 August 2003

Yersinia enterocolitica synthesizes N-acyl-L-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-L-homoserine lactone] and not HHL (N-hexanoyl-L-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.

* Corresponding author. Mailing address: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillian University Munich, Pettenkoferstrasse 9a, 80336 Munich, Germany. Phone: (49-89) 51605227. Fax: (49-89) 51605223. E-mail: jacobi{at}m3401.mpk.med.uni-muenchen.de.

Editor: B. B. Finlay

Infection and Immunity, November 2003, p. 6624-6626, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6624-6626.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2003 by the American Society for Microbiology. All rights reserved.