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Infection and Immunity, December 2003, p. 7208-7210, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7208-7210.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells

Nathan A. Groathouse,1,2 Robert A. Heinzen,1,3 and Scott Boitano1,4*

Department of Zoology and Physiology and Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82701,1 Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-1682,2 Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840,3 Department of Physiology, Arizona Respiratory Center, University of Arizona, Tucson, Arizona 85724-50304

Received 18 August 2003/ Returned for modification 2 September 2003/ Accepted 9 September 2003

To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.

* Corresponding author. Mailing address: Arizona Health Sciences Center, University of Arizona, Room 2338, AHSC, 1501 N. Campbell Ave., Tucson, AZ 85724-5030. Phone: (520) 626-2105. Fax: (520) 626-6970. E-mail: sboitano{at}email.arizona.edu.

Editor: D. L. Burns

Infection and Immunity, December 2003, p. 7208-7210, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7208-7210.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Edwards, J. A., Groathouse, N. A., Boitano, S. (2005). Bordetella bronchiseptica Adherence to Cilia Is Mediated by Multiple Adhesin Factors and Blocked by Surfactant Protein A. Infect. Immun. 73: 3618-3626 [Abstract] [Full Text]  
  • Anderton, T. L., Maskell, D. J., Preston, A. (2004). Ciliostasis is a key early event during colonization of canine tracheal tissue by Bordetella bronchiseptica. Microbiology 150: 2843-2855 [Abstract] [Full Text]  


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