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Infection and Immunity, February 2003, p. 794-800, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.794-800.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Colonization of C57BL/6J and BALB/c Wild-Type and Knockout Mice with Helicobacter pylori: Effect of Vaccination and Implications for Innate and Acquired Immunity

Klaus Panthel,1 Gerhard Faller,2 and Rainer Haas1*

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Munich,1 Institut für Pathologie, Universität Erlangen-Nürnberg, Erlangen, Germany2

Received 15 October 2002/ Accepted 6 November 2002

The gram-negative bacterial pathogen Helicobacter pylori is a major cause of peptic ulcer disease and a risk factor for gastric cancer in humans. Adapted H. pylori strains, such as strain SS1, are able to infect mice and are a useful model for gastric colonization and vaccination studies. In this study we used a streptomycin-resistant derivative of H. pylori SS1 to analyze the colonization behavior and the success of vaccination in wild-type (wt) and various knockout mice of the BALB/c and C57BL/6J genetic backgrounds. We here report that BALB/c interleukin-4 knockout (IL-4-/-) mice are weakly overcolonized compared to the wt strain but that the IL-12-/- knockout results in a strong overcolonization (500%). Unexpectedly, in the C57BL/6J background the same knockouts behaved in diametrically opposed manners. The IL-4-/- mutation caused a 50% reduction and the IL-12-/- knockout caused a 95% reduction compared to the wt colonization rate. For C57BL/6J mice we further analyzed the IL-18-/- and Toll-like receptor 2 knockout mutations, which showed reductions to 66 and 57%, respectively, whereas mice with the IL-10-/- phenotype were hardly infected at all (5%). In contrast, the tumor necrosis factor receptor knockout (p55-/- and p55/75-/-) mice showed an overcolonization compared to the C57BL/6J wt strain. With exception of the low-level infected C57BL/6J IL-10-/- and IL-12-/- knockout mice, all knockout mutants were accessible to a prophylactic vaccination and their vaccination behavior was comparable to that of the wt strains.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: (49)-89-51605255. Fax: (49)-89-51605223. E-mail: haas{at}m3401.mpk.med.uni-muenchen.de.

Editor: D. L. Burns

Infection and Immunity, February 2003, p. 794-800, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.794-800.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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