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Infection and Immunity, March 2003, p. 1557-1560, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1557-1560.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Studies of the Mechanism of Action of the Aerolysin-Like Hemolysin of Aeromonas sobria in Stimulating T84 Cells To Produce Cyclic AMP

Yoshio Fujii,1* Tomohiko Nomura,2 Ritsuko Yokoyama,1 Sumio Shinoda,3 and Keinosuke Okamoto3

Institute of Pharmacognosy,1 Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima 770-8514,2 Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Okayama, Okayama 700-8530, Japan3

Received 3 July 2002/ Returned for modification 11 September 2002/ Accepted 12 November 2002

We previously reported that the aerolysin-like hemolysin of Aeromonas sobria stimulates T84 cells to produce cyclic AMP, which then emerges in the culture medium. In order to clarify the mechanism of action of the hemolysin, we examined the involvement of adenosine nucleotide. The results show that the hemolysin stimulates T84 cells to release ATP, which is then converted to adenosine by ectonucleotidase. The adenosine generated might stimulate the P1 adenosine receptors of T84 cells to produce cyclic AMP.

* Corresponding author. Mailing address: Institute of Pharmacognosy, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima 770-8514, Japan. Phone: 81-88-622-9611, x5701. Fax: 81-88-655-3051. E-mail: fujii{at}ph.bunri-u.ac.jp.

Editor: B. B. Finlay

Infection and Immunity, March 2003, p. 1557-1560, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1557-1560.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fujii, Y., Tsurumi, K., Sato, M., Takahashi, E., Okamoto, K. (2008). Fluid Secretion Caused by Aerolysin-Like Hemolysin of Aeromonas sobria in the Intestines Is Due to Stimulation of Production of Prostaglandin E2 via Cyclooxygenase 2 by Intestinal Cells. Infect. Immun. 76: 1076-1082 [Abstract] [Full Text]  
  • Crane, J. K., Naeher, T. M., Choudhari, S. S., Giroux, E. M. (2005). Two pathways for ATP release from host cells in enteropathogenic Escherichia coli infection. Am. J. Physiol. Gastrointest. Liver Physiol. 289: G407-G417 [Abstract] [Full Text]  
  • Epple, H. J., Mankertz, J., Ignatius, R., Liesenfeld, O., Fromm, M., Zeitz, M., Chakraborty, T., Schulzke, J. D. (2004). Aeromonas hydrophila Beta-Hemolysin Induces Active Chloride Secretion in Colon Epithelial Cells (HT-29/B6). Infect. Immun. 72: 4848-4858 [Abstract] [Full Text]  


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