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Infection and Immunity, April 2003, p. 2065-2070, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.2065-2070.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Exposure of Immunocompetent Adult Mice to Pneumocystis carinii f. sp. muris by Cohousing: Growth of P. carinii f. sp. muris and Host Immune Response

Chun Li An,1,{dagger} Francis Gigliotti,2 and Allen G. Harmsen1*

Trudeau Institute, Saranac Lake, New York 12983,1 Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York 146422

Received 5 August 2002/ Returned for modification 10 September 2002/ Accepted 13 January 2003

There has been emerging evidence that immunocompetent hosts can harbor Pneumocystis in their lungs. The purpose of this study was to determine the kinetics of Pneumocystis carinii f. sp. muris infection in adult immunocompetent mice and the host immune response to the organisms. To accomplish this, we exposed adult immunocompetent mice to SCID mice infected with P. carinii f. sp. muris by cohousing. We found that P. carinii f. sp. muris was detectable in the lungs of cohoused immunocompetent mice by PCR by 3 weeks after the beginning of cohousing. At about 4 weeks of cohousing, P. carinii f. sp. muris was readily detectable in the lungs of mice by microscopic techniques. Also at this time, P. carinii f. sp. muris-specific immunoglobulin G was found in the sera of the mice, and CD62low CD4- and CD8-positve T cells accumulated in the lungs. Shortly after this immune response, the P. carinii f. sp. muris organisms were cleared from the lungs. Adult mice cohoused for only 1 week also contained P. carinii f. sp. muris cysts detectable by silver staining at 5 and 6 weeks after the beginning of cohousing. We also found that the P. carinii f. sp. muris organisms grew to greater numbers in the lungs of BALB/c mice than in those of C57BL6 mice. This indicates that immunocompetent hosts develop a mild infection with P. carinii f. sp. muris which resolves in 5 to 6 weeks when there is a detectable immune response to the organism. Once an acquired immune response was initiated, the P. carinii f. sp. muris organisms were quickly eliminated without clinical signs of disease.

* Corresponding author. Present address: Veterinary Molecular Biology, P.O. Box 173610, Montana State University, Bozeman, MT 59717-3610. Phone: (406) 994-4706. Fax: (406) 994-4303. E-mail: aharmsen{at}montana.edu.

Editor: T. R. Kozel

{dagger} Present address: Pathogen Biology, China Medical University, Shenyang, People's Republic of China.

Infection and Immunity, April 2003, p. 2065-2070, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.2065-2070.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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