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Infection and Immunity, May 2003, p. 2626-2633, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2626-2633.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Induction of Proinflammatory Responses in the Human Monocytic Cell Line THP-1 by Campylobacter jejuni

Michael A. Jones,1* Sabine Tötemeyer,2 Duncan J. Maskell,2 Clare E. Bryant,2 and Paul A. Barrow1

Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN,1 Centre for Veterinary Sciences, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom2

Received 4 November 2002/ Returned for modification 19 December 2002/ Accepted 10 February 2003

Campylobacter jejuni can cause an enteritis that is associated with an acute inflammatory response at the gut epithelial surface. The signals inducing inflammation are unknown. C. jejuni can penetrate the intestinal epithelial barrier and may then interact with leucocytes, potentially inducing proinflammatory responses. To investigate this, we studied the interaction of C. jejuni with the human monocytic cell line THP-1 and show that a range of proinflammatory cytokines and chemokines is induced. These include interleukin-1{alpha} (IL-1{alpha}), IL-1ß, IL-6, IL-8, and tumor necrosis factor alpha. Responses can be induced by killed Campylobacter as well as live bacteria and do not depend on the cytolethal distending toxin. C. jejuni infection of THP-1 cells triggers both nuclear translocation of functional NF-{kappa}B and stimulation of IL-1{alpha}, indicating that NF-{kappa}B-dependent and -independent stimulation is occurring. The extent of proinflammatory cytokine stimulation suggests that monocytes might significantly contribute to intestinal inflammation and disease pathology.

* Corresponding author. Mailing address: Institute for Animal Health, High St., Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 1635 578411. Fax: 1635 577243. E-mail: majones{at}bbsrc.ac.uk.

Editor: B. B. Finlay

Infection and Immunity, May 2003, p. 2626-2633, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2626-2633.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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