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Infection and Immunity, May 2003, p. 2902-2906, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2902-2906.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Influence of Lewis Antigen Expression by Helicobacter pylori on Bacterial Internalization by Gastric Epithelial Cells

Alain Lozniewski,1* Xavier Haristoy,1 David A. Rasko,2 Renée Hatier,3 François Plénat,4 Diane E. Taylor,5 and Karine Angioi-Duprez6

Laboratoire de Bactériologie-Virologie,1 EMI INSERM 0014, Laboratoire de Microscopie Electronique,3 Laboratoire d'Anatomie Pathologique,4 Laboratoire de Microchirurgie Expérimentale, Faculté de Médecine, Université Henri-Poincaré, Vandoeuvre-les-Nancy, France,6 The Institute for Genomic Research, Rockville, Maryland,2 Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada5

Received 1 August 2002/ Returned for modification 4 November 2002/ Accepted 23 January 2003

The role of Helicobacter pylori lipopolysaccharide (LPS) Lewis antigens in infection is still not well known. We investigated the influence of Lewis antigen expression by H. pylori on its internalization by AGS cells and the epithelium of human gastric xenografts in nude mice using isogenic mutants in LPS biosynthetic genes. In vivo, colonization rates were unaffected by the change in H. pylori Lewis antigen expression, whereas the number of viable intracellular bacteria was significantly higher with wild-type H. pylori strains expressing Lewis antigens when compared to the isogenic mutants in both models. H. pylori strains expressing more Lewis X antigens (Lex) were internalized at a higher rate than those expressing less Lex, type II Lewis antigens (Lea or Leb) alone, or no Lewis antigens. Thus, Lewis antigens appear to be involved in the internalization of H. pylori by the gastric epithelium.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Central, 29 Ave. du Maréchal de Lattre de Tassigny, 54035 Nancy Cedex, France. Phone: (33) 3 83 85 21 96. Fax: (33) 3 83 85 26 73. E-mail: a.lozniewski{at}chu-nancy.fr.

Editor: V. J. DiRita

Infection and Immunity, May 2003, p. 2902-2906, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2902-2906.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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