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Infection and Immunity, May 2003, p. 2966-2969, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2966-2969.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Immunization with Genetic Toxoids of the Arcanobacterium pyogenes Cholesterol-Dependent Cytolysin, Pyolysin, Protects Mice against Infection

B. Helen Jost,* Hien T. Trinh, J. Glenn Songer, and Stephen J. Billington

Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona 85721

Received 21 October 2002/ Returned for modification 18 December 2002/ Accepted 21 January 2003

Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F497, HIS-PLO.{Delta}P499, and HIS-PLO.A522, were found to be nontoxic, and vaccinated mice were protected from infection, indicating the potential of these toxoids as vaccines. Furthermore, in a mouse model of infection, A. pyogenes carrying the F497 mutation was as attenuated as a PLO-deficient strain, indicating that the cytolytic activity of PLO is important in virulence.

* Corresponding author. Mailing address: Department of Veterinary Science and Microbiology, The University of Arizona, 1117 East Lowell St., Tucson, AZ 85721. Phone: (520) 621-2745. Fax: (520) 621-6366. E-mail: jost{at}u.arizona.edu.

Editor: A. D. O'Brien

Infection and Immunity, May 2003, p. 2966-2969, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2966-2969.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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