This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hsieh, Y.-C.
Right arrow Articles by Liang, C.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsieh, Y.-C.
Right arrow Articles by Liang, C.-M.

 Previous Article  |  Next Article 

Infection and Immunity, June 2003, p. 3329-3336, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3329-3336.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Study of Capsular Polysaccharide from Vibrio parahaemolyticus

Yu-Chi Hsieh,1 Shu-Mei Liang,2 Wan-Ling Tsai,3 Yee-Hsiung Chen,1 Teh-Yung Liu,1 and Chi-Ming Liang1*

Institute of Biological Chemistry,1 Institute of Bioagricultural Sciences,2 Office of Public Affairs, Academia Sinica, Taipei, Taiwan 115293

Received 30 October 2002/ Returned for modification 6 January 2003/ Accepted 7 March 2003

The leading cause of food poisoning in both Taiwan and Japan is Vibrio parahaemolyticus infection, whose mechanism of enteropathogenesis is still unclear. To evaluate whether surface components are responsible for the intestinal adhesion of V. parahaemolyticus, we have developed a novel method for isolating the capsular polysaccharide (CPS) from V. parahaemolyticus (serotype O4:K8). We found that culturing of V. parahaemolyticus in broth for 1 week or more changed the colony form of the bacteria on an agar plate from opaque to translucent. The translucent colonies of V. parahaemolyticus contained little CPS and exhibited a much lower level of adherence to epithelial cells (Int-407) than the opaque colonies of the bacteria. Incubation of V. parahaemolyticus in medium supplemented with bile increased the levels of CPS and adherence. Treatment of V. parahaemolyticus with anti-CPS but not anti-LPS serum decreased the level of bacterial adherence. In addition, purified CPS bound to epithelial cells in a dose-dependent manner. Intranasal administration of CPS to mice in the presence of adjuvants such as immunostimulatory sequence oligodeoxynucleotides or cholera toxin elicited CPS-specific mucosal and systemic immune responses. These results indicate that CPS plays an important role in the adherence of V. parahaemolyticus to its target cells and may be considered a potential target for the development of a vaccine against this pathogen.

* Corresponding author. Mailing address: Institute of Biological Chemistry, Academia Sinica, No. 128, Academia Rd., Section 2, Taipei, Taiwan 11529. Phone: 886227899383, ext. 101. Fax: 886226518049. E-mail: cmliang{at}gate.sinica.edu.tw.

Editor: J. D. Clements

Infection and Immunity, June 2003, p. 3329-3336, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3329-3336.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Audy, J., Labrie, S., Roy, D., LaPointe, G. (2010). Sugar source modulates exopolysaccharide biosynthesis in Bifidobacterium longum subsp. longum CRC 002. Microbiology 156: 653-664 [Abstract] [Full Text]  
  • Jeong, H. G., Oh, M. H., Kim, B. S., Lee, M. Y., Han, H. J., Choi, S. H. (2009). The Capability of Catabolic Utilization of N-Acetylneuraminic Acid, a Sialic Acid, Is Essential for Vibrio vulnificus Pathogenesis. Infect. Immun. 77: 3209-3217 [Abstract] [Full Text]  
  • Ruas-Madiedo, P., Gueimonde, M., Arigoni, F., de los Reyes-Gavilan, C. G., Margolles, A. (2009). Bile Affects the Synthesis of Exopolysaccharides by Bifidobacterium animalis. Appl. Environ. Microbiol. 75: 1204-1207 [Abstract] [Full Text]  
  • Jaques, S., McCarter, L. L. (2006). Three New Regulators of Swarming in Vibrio parahaemolyticus. J. Bacteriol. 188: 2625-2635 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»