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Infection and Immunity, June 2003, p. 3634-3638, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3634-3638.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cytolethal Distending Toxin Gene Cluster in Enterohemorrhagic Escherichia coli O157:H- and O157:H7: Characterization and Evolutionary Considerations

Andreas Janka,1 Martina Bielaszewska,1 Ulrich Dobrindt,2 Lilo Greune,3 M. Alexander Schmidt,3 and Helge Karch1*

Institut für Hygiene, Universitätsklinikum Münster,1 Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Universität Münster, 48149 Münster,3 Institut für Molekulare Infektionsbiologie, Universität Würzburg, 97070 Würzburg, Germany2

Received 8 November 2002/ Returned for modification 26 February 2003/ Accepted 18 March 2003

We identified a cytolethal distending toxin (cdt) gene cluster in 87, 6, and 0% of sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H-, EHEC O157:H7, and E. coli O55:H7/H- strains, respectively. The toxin was expressed by the wild-type EHEC O157 strains and by a cdt-containing cosmid from a library of SF EHEC O157:H- strain 493/89. The cdt flanks in strain 493/89 were homologous to bacteriophages P2 and lambda. Our data demonstrate that cdt, encoding a potential virulence factor, is present in the EHEC O157 complex and suggest that cdt may have been acquired by phage transduction.

* Corresponding author. Mailing address: Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch Str. 41, 48149 Münster, Germany. Phone: 49-251-8355361. Fax: 49-251-8355341. E-mail: hkarch{at}uni-muenster.de.

Editor: A. D. O'Brien

Infection and Immunity, June 2003, p. 3634-3638, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3634-3638.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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