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Infection and Immunity, September 2003, p. 4842-4849, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.4842-4849.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

MtuA, a Lipoprotein Receptor Antigen from Streptococcus uberis, Is Responsible for Acquisition of Manganese during Growth in Milk and Is Essential for Infection of the Lactating Bovine Mammary Gland

Amanda J. Smith, Philip N. Ward, Terence R. Field, Catherine L. Jones, Ruth A. Lincoln, and James A. Leigh*

Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berks RG20 7NN, United Kingdom

Received 4 February 2003/ Returned for modification 16 April 2003/ Accepted 2 June 2003

A mutant strain of Streptococcus uberis (AJS001) that was unable to grow in bovine milk was isolated following random insertional mutagenesis. The level of growth in milk was restored to that of the parental strain (strain 0140J) following addition of MnSO4 but not following addition of other metal ions. The mutant contained a single insertion within mtuA, a homologue of mtsA and psaA, which encode metal-binding proteins in Streptococcus pyogenes and Streptococcus pneumoniae, respectively. Strain AJS001 was unable to infect any of eight quarters on four dairy cows following intramammary challenge with 105 CFU. Bacteria were never recovered directly from milk of these animals but were detected following enrichment in Todd-Hewitt broth in three of eight milk samples obtained within 24 h of challenge. The animals showed no inflammatory response and no signs of mastitis. Three mammary quarters on two different animals simultaneously challenged with 600 CFU of the parental strain, strain 0140J, became colonized, shed high numbers of S. uberis organisms in milk, displayed a marked inflammatory response to infection, and showed overt signs of mastitis. These data indicate that mtuA was required for efficient uptake of Mn2+ during growth in bovine milk and infection of the lactating bovine mammary gland.

* Corresponding author. Mailing address: Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berks RG 20 7NN, United Kingdom. Phone: 4 1635 577249. Fax: 4 1635 577243. E-mail: james.leigh{at}bbsrc.ac.uk.

Editor: A. D. O'Brien

Infection and Immunity, September 2003, p. 4842-4849, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.4842-4849.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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