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Infection and Immunity, September 2003, p. 4857-4861, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.4857-4861.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus Induces Protective Immunity in BALB/c Mice

Angel A. Oñate,1* Sandra Céspedes,1 Alex Cabrera,1 Rodolfo Rivers,1 Andrés González,1 Carola Muñoz,1 Hugo Folch,2 and Edilia Andrews1

Molecular Immunology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción,1 Department of Immunology, Faculty of Medicine, Universidad Austral, Valdivia, Chile2

Received 30 January 2003/ Returned for modification 7 May 2003/ Accepted 29 May 2003

This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, P.O. Box 152-C, Concepción, Chile. Phone: (056) 41-204118. Fax: (056) 41-245975. E-mail: aonate{at}udec.cl.

Editor: J. D. Clements

Infection and Immunity, September 2003, p. 4857-4861, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.4857-4861.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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